Ginkgolide B increases hydrogen sulfide and protects against endothelial dysfunction in diabetic rats.
Ginkgolide B increases hydrogen sulfide and protects against endothelial dysfunction in diabetic rats.
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DOI：
10.3325/cmj.2015.56.4
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发表时间：
2015-02
影响因子：
1.9
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Wang GG;Chen QY;Li W;Lu XH;Zhao X
目的：探讨银杏内酯B对糖尿病大鼠血管内皮功能的影响。将15只雄性SD大鼠分为4组：对照组、银杏内酯B组、糖尿病组和银杏内酯B组，测定主动脉组织中超氧化物歧化酶(SOD)活性、丙二醛含量、NADPH氧化酶亚基和谷胱甘肽过氧化物酶1(GPX1)蛋白的表达。观察主动脉环对苯肾上腺素(Phe)的收缩反应和对乙酰胆碱(Ach)和硝普钠(SNP)的收缩反应。检测一氧化氮(NO)、硫化氢(H 2S)含量、胱硫醚γ裂解酶(CSE)和胱硫醚β合成酶(CBS)蛋白表达及内皮型一氧化氮合酶(ENOS)活性。糖尿病显著抑制苯丙氨酸诱导的血管收缩和乙酰胆碱诱导的血管松弛(P <； 0.001)，降低NO生物利用度和硫化氢生成(P <； 0.001)，降低超氧化物歧化酶活性和GPX1蛋白表达(P <； 0.001)，增加丙二醛含量和NADPH氧化酶亚基，以及CSE和Cbs蛋白表达(P <； 0.001)。银杏内酯B可改善Phe血管收缩和Ach血管松弛(P <； 0.001)，恢复超氧化物歧化酶(P = 0.005)和内皮型一氧化氮合酶(P <； 0.001)活性，减少硫化氢生成(P = 0.044)，降低丙二醛含量(P = 0.014)。正常大鼠和糖尿病大鼠经银杏内酯B治疗后，对硝普钠的血管松弛作用无显著差异(fi)。此外，银杏内酯B增加了GPX1蛋白的表达，降低了NADPH氧化酶亚基、CBS和CSE蛋白的表达。银杏内酯B通过减少氧化应激，提高NO生物利用度和硫化氢的产生，减轻糖尿病大鼠的内皮功能障碍。
To evaluate the effect of ginkgolide B treatment on vascular endothelial function in diabetic rats. The study included four groups with 15 male Sprague-Dawley rats: control group; control group treated with ginkgolide B; diabetic group; and diabetic treated with ginkgolide B. The activity of superoxide dismutase (SOD), malondialdehyde content, and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits, and glutathione peroxidase 1 (GPX1) protein expression were determined in aortic tissues. Vasoconstriction to phenylephrine (PHE) and vasorelaxation to acetylcholine (Ach) and sodium nitroprusside (SNP) were assessed in aortic rings. Nitric oxide (NO) and hydrogen sulfide (H2S) were measured, as well as cystathionine γ lyase (CSE) and cystathionine β synthetase (CBS) protein expression, and endothelial nitric oxide synthase (eNOS) activity. Diabetes significantly impaired PHE-induced vasoconstriction and Ach-induced vasorelaxation (P < 0.001), reduced NO bioavailability and H2S production (P < 0.001), SOD activity, and GPX1 protein expression (P < 0.001), and increased malondialdehyde content and NADPH oxidase subunits, and CSE and CBS protein expression (P < 0.001). Ginkgolide B treatment improved PHE vasoconstriction and Ach vasorelaxation (P < 0.001), restored SOD (P = 0.005) and eNOS (P < 0.001) activities, H2S production (P = 0.044) and decreased malondialdehyde content (P = 0.014). Vasorelaxation to SNP was not significantly different in control and diabetic rats with or without ginkgolide B treatment. Besides, ginkgolide B increased GPX1 protein expression and reduced NADPH oxidase subunits, CBS and CSE protein expression. Ginkgolide B alleviates endothelial dysfunction by reducing oxidative stress and elevating NO bioavailability and H2S production in diabetic rats.
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影响因子：
37.8
作者：
Calvert JW;Elston M;Nicholson CK;Gundewar S;Jha S;Elrod JW;Ramachandran A;Lefer DJ
通讯作者：
Lefer DJ
DOI：
10.1124/jpet.105.092023
发表时间：
2006-02-01
影响因子：
3.5
作者：
Bian, JS;Yong, QC;Moore, PK
通讯作者：
Moore, PK
影响因子：
37.8
作者：
Eckel, Robert H.;Kahn, Richard;Rizza, Robert A.
通讯作者：
Rizza, Robert A.
影响因子：
3.5
作者：
Li, Rui;Chen, Beidong;Qi, Ruomei
通讯作者：
Qi, Ruomei
影响因子：
120.1
作者：
Drummond, Grant R.;Selemidis, Stavros;Griendling, Kathy K.;Sobey, Christopher G.
通讯作者：
Sobey, Christopher G.