Plasma derived from human umbilical cord blood: Potential cell-additive or cell-substitute therapeutic for neurodegenerative diseases.
Plasma derived from human umbilical cord blood: Potential cell-additive or cell-substitute therapeutic for neurodegenerative diseases.
复制标题
DOI：
10.1111/jcmm.13898
复制
发表时间：
2018-12
影响因子：
5.3
通讯作者：
Garbuzova-Davis S
中科院分区：
文献类型：
--
作者：
Ehrhart J;Sanberg PR;Garbuzova-Davis S
目前神经退行性疾病的治疗方法疗效有限，导致人们对替代疗法的兴趣增加。从人脐带血（human umbilical cord blood，hUCB）中提取的脐血血浆（cord blood plasma，CBP）可能是一种潜在的治疗药物。CBP注射到衰老或缺血性中风的啮齿动物模型中的益处已得到证实，但如何产生益处仍不清楚。本研究评价了CBP和成人血浆/血清（ABP/S）相同样品中的各种因素。此外，还测定了自体CBP与ABP/S或胎牛血清补充剂对hUCB单核细胞（MNC hUCB）的体外作用。结果显示CBP中促炎细胞因子（IL-2、IL-6、IFN-γ和TNF-α）浓度显著降低，趋化因子IL-8升高。CBP中测定的VEGF、G-CSF、EGF和FGF-碱性生长因子水平显著高于ABP/S。AutoblastCBP培养基补充物显著增加MNC hUCB活力并降低凋亡细胞活性。我们首次证明了在源自hUCB的相同CBP样品中细胞因子和生长因子的独特CBP组成。此外，我们的新发现，自体CBP促进MNC hUCB活力和减少体外凋亡细胞死亡，支持CBP作为开发各种神经退行性疾病疗法的唯一治疗剂或细胞添加剂的潜力。
Limited efficacy of current therapeutic approaches for neurodegenerative disease has led to increased interest in alternative therapies. Cord blood plasma (CBP) derived from human umbilical cord blood (hUCB) may be a potential therapeutic. Benefits of CBP injection into rodent models of aging or ischaemic stroke have been demonstrated, though how benefits are elicited is still unclear. The present study evaluated various factors within the same samples of CBP and human adult blood plasma/sera (ABP/S). Also, autologous CBP effects vs. ABP/S or foetal bovine serum supplements on mononuclear cells from hUCB (MNC hUCB) in vitro were determined. Results showed significantly low concentrations of pro‐inflammatory cytokines (IL‐2, IL‐6, IFN‐γ, and TNF‐α) and elevated chemokine IL‐8 in CBP. Significantly higher levels of VEGF, G‐CSF, EGF and FGF‐basic growth factors were determined in CBP vs. ABP/S. Autologous CBP media supplements significantly increased MNC hUCB viability and decreased apoptotic cell activity. We are first to demonstrate the unique CBP composition of cytokines and growth factors within the same CBP samples derived from hUCB. Also, our novel finding that autologous CBP promoted MNC hUCB viability and reduced apoptotic cell death in vitro supports CBP's potential as a sole therapeutic or cell‐additive agent in developing therapies for various neurodegenerative diseases.
登录
查看更多内容
DOI：
10.5114/aoms.2013.39237
发表时间：
2013-12-30
期刊：
Archives of medical science : AMS
影响因子：
--
作者：
Abo-Grisha N;Essawy S;Abo-Elmatty DM;Abdel-Hady Z
通讯作者：
Abdel-Hady Z
影响因子：
3.3
作者：
Boltze, Johannes;Schmidt, Uwe R.;Schaebitz, Wolf-Ruediger
通讯作者：
Schaebitz, Wolf-Ruediger
影响因子：
3.7
作者：
Acosta, Sandra A.;Tajiri, Naoki;Borlongan, Cesar V.
通讯作者：
Borlongan, Cesar V.
影响因子：
2.8
作者：
Denihan, N. M.;Looney, A. M.;Murray, D. M.
通讯作者：
Murray, D. M.
影响因子：
3.3
作者：
Eve, David J.;Ehrhart, Jared;Garbuzova-Davis, Svitlana
通讯作者：
Garbuzova-Davis, Svitlana