Effects of intravenous human umbilical cord blood CD34+ stem cell therapy versus levodopa in experimentally induced Parkinsonism in mice.
Effects of intravenous human umbilical cord blood CD34+ stem cell therapy versus levodopa in experimentally induced Parkinsonism in mice.
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DOI：
10.5114/aoms.2013.39237
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发表时间：
2013-12-30
期刊：
影响因子：
--
通讯作者：
Abdel-Hady Z
中科院分区：
文献类型：
--
作者：
Abo-Grisha N;Essawy S;Abo-Elmatty DM;Abdel-Hady Z
帕金森综合征是一种运动功能受损的神经退行性疾病。本研究旨在探讨CD 34+干细胞与左旋多巴对1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）诱导的帕金森病的影响。将小鼠分成4组;盐水注射，MPTP：接受四次MPTP注射（20 mg/kg，i. p.）MPTP组在第7天以2小时间隔用左旋多巴/卡比多巴（100/10 mg/kg/两次/天，持续28天）或单次静脉内注射106个CD 34+干细胞/小鼠处理，并使其存活至第5周结束。左旋多巴和干细胞改善MPTP诱导的运动缺陷;它们消除了帕金森病小鼠的步幅差异，降低了脚滑错误百分比，并增加了行走、活动系数和移动持续时间（p < 0.05）。此外，与MPTP（分别为60.1 ±3.9 pmol/g和3.6 ±0.09 mmol/g）相比，它们显著（p <0.05）增加了纹状体多巴胺（85.3 ±4.3和110.6 ±5.3）和ATP水平（10.6 ±1.1和15.5 ±1.14）（p < 0.05）。此外，与MPTP组相比，左旋多巴或干细胞处理的小鼠的线粒体DNA处于完整形式;平均浓度为（52.8 ±3.01和107.8 ±8.6），未发现明显的核DNA断裂。在酪氨酸羟化酶（TH）免疫染色方面，干细胞组TH阳性神经元的百分比（63.55 ±5.2）明显高于MPTP组（37.6 ±3.1）和左旋多巴组（41.6 ±3.5）。CD 34+细胞改善MPTP-帕金森病小鼠的运动、生化和组织学缺陷，这些效果上级于左旋多巴产生的效果，这将有望用于治疗PD。
Parkinsonism is a neurodegenerative disease with impaired motor function. The current research was directed to investigate the effect of CD34+ stem cells versus levodopa in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinsonism. Mice were divided into 4 groups; saline-injected, MPTP: received four MPTP injections (20 mg/kg, i.p.) at 2 h intervals, MPTP groups treated with levodopa/carbidopa (100/10 mg/kg/twice/day for 28 days) or single intravenous injection of 106 CD34+ stem cells/mouse at day 7 and allowed to survive until the end of week 5. Levodopa and stem cells improved MPTP-induced motor deficits; they abolished the difference in stride length, decreased percentage of foot slip errors and increased ambulation, activity factor and mobility duration in parkinsonian mice (p < 0.05). Further, they significantly (p < 0.05) increased striatal dopamine (85.3 ±4.3 and 110.6 ±5.3) and ATP levels (10.6 ±1.1 and 15.5 ±1.14) compared to MPTP (60.1 ±3.9 pmol/g and 3.6 ±0.09 mmol/g, respectively) (p < 0.05). Moreover, mitochondrial DNA from mice treated with levodopa or stem cells was in intact form; average concentration was (52.8 ±3.01 and 107.8 ±8.6) and no appreciable fragmentation of nuclear DNA was found compared to MPTP group. Regarding tyrosine hydroxylase (TH) immunostaining, stem cell group showed a marked increase of percentage of TH-immunopositive neurons (63.55 ±5.2) compared to both MPTP (37.6 ±3.1) and levodopa groups (41.6 ±3.5). CD34+ cells ameliorated motor, biochemical and histological deficits in MPTP-parkinsonian mice, these effects were superior to those produced by levodopa that would be promising for the treatment of PD.
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影响因子：
14.8
作者：
Jackson-Lewis, Vernice;Przedborski, Serge
通讯作者：
Przedborski, Serge
影响因子：
7.4
作者：
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影响因子：
5.1
作者：
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影响因子：
6.5
作者：
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4.4
作者：
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