Fragmentation patterns of boron-dipyrromethene (BODIPY) dyes by electrospray ionization high-resolution tandem mass spectrometry.
Fragmentation patterns of boron-dipyrromethene (BODIPY) dyes by electrospray ionization high-resolution tandem mass spectrometry.
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通过电喷雾电离高分辨率串联质谱法观察硼二吡咯亚甲基 (BODIPY) 染料的裂解模式
DOI：
10.1002/rcm.7179
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
D. Volmer
中科院分区：
文献类型：
--
作者：
T. Geib;A.-M. Huynh;G. Jung;D. Volmer
Rationale4，4‐Difluoro‐4‐bora‐3a，4a‐diaza‐s‐indacene衍生物(BODIPY)是一种荧光有机染料，在生物分析中被广泛用作非放射性标记。方法采用电喷雾电离(ESI)-MS/MS联用技术和高分辨、准确的傅立叶变换离子回旋共振(FTICR)实验数据，对10个结构相关的BODIPY进行电喷雾电离(ESI)-MS/MS研究。在随后的CID实验中，这些自由基物种解离成大范围的产物离子。平行的[M]+·和[M+H]+分解产生的叠加解离使MS/MS图谱的解释变得非常复杂。结论本研究提出了BODIPY染料的解离机理。使用FTICR-MS明确指定了CID产物离子的元素式，并发现了独特的碎片离子，用于通过低分辨率MS快速鉴定BODIPY合成混合物中单个染料的甲基、乙基、丁基、叔丁基和苯基取代基。版权所有©2015 John Wiley&Sons，Ltd.
Rationale4,4‐Difluoro‐4‐bora‐3a,4a‐diaza‐s‐indacene derivatives (BODIPYs) are fluorescent organic dyes that are widely used as non‐radioactive labels in biological analyses. The fragmentation behaviour of ten structurally related BODIPYs was studied using tandem mass spectrometry (MS/MS), to support the structural elucidation process during synthesis.MethodsThe BODIPYs were investigated by electrospray ionization (ESI)‐MS/MS, utilizing collision‐induced dissociation (CID) data from triple quadrupole MS and high‐resolution, accurate mass CID data from Fourier transform ion cyclotron resonance (FTICR) experiments.ResultsUnusual radical molecular cations ([M]+•) were formed directly during the ESI process. These radical species dissociated into a large range of product ions during the subsequent CID experiments. Superimposed dissociations originating from parallel [M]+•and [M+H]+decompositions significantly complicated the interpretation of the MS/MS spectra.ConclusionsDetailed dissociation mechanisms were proposed in this study for BODIPY dyes. The elemental formulae of CID product ions were unambiguously assigned using FTICR‐MS and unique fragment ions were discovered for the rapid identification of methyl, ethyl, butyl,tert‐butyl, and phenyl substituents of individual dyes in BODIPY synthesis mixtures by low‐resolution MS. Copyright © 2015 John Wiley & Sons, Ltd.
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荧光光谱氧化反应的机理研究：关键评估
DOI：
--
发表时间：
2009
期刊：
影响因子：
--
作者：
Alexander Schmitt;Babette Hinkeldey;Benjamin Hötzer;G. Jung
通讯作者：
G. Jung
影响因子：
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作者：
Mosely, Jackie A.;Smith, Michael J. P.;Bristow, Anthony W. T.
通讯作者：
Bristow, Anthony W. T.
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影响因子：
3.2
作者：
Bortoli S;Volmer DA
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Volmer DA
影响因子：
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作者：
Florian, Alexander;Mayoral, Maria Jose;Fernandez, Gustavo
通讯作者：
Fernandez, Gustavo
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影响因子：
3.2
作者：
Juan Wei;Huilin Li;M. Barrow;P. O’Connor
通讯作者：
P. O’Connor