High-sensitivity amino acid analysis of stained peptides and proteins from a sodium dodecyl sulphate-polyacrylamide slab gel.
High-sensitivity amino acid analysis of stained peptides and proteins from a sodium dodecyl sulphate-polyacrylamide slab gel.
复制标题
对十二烷基硫酸钠-聚丙烯酰胺平板凝胶中的染色肽和蛋白质进行高灵敏度氨基酸分析。
DOI：
10.1016/s0021-9673(01)87050-3
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Y. Kubota
中科院分区：
文献类型：
--
作者：
S. Shoji;M. Ichikawa;T. Yamaoka;T. Funakoshi;Y. Kubota
PTC-amino acids, especially PTC-proline and phenylthiourea, and to apply it. to the amino acid analysis of acid hydrolysates of insulin B-chain, insulin, cytochrome c and other peptides and proteins extracted from a polyacrylamide slab gel after electrophoresis and staining with Coomassie brilliant blue. They were considered to produce a large amount of ammonium chloride on acid hydrolysis. We achieved their separation by the addition of 0.1% of sodium dodecyl sulphate (SDS) to the mobile phase. The results of amino acid analysis showed that the numbers of amino acids in these samples were each close to those calculated from their amino acid sequences, with a few exceptions. This method may be suitable for the routine microanalysis of the hydrolysates of peptides and proteins which are separated and stained on SDS-polyacrylamide slab gels,
高灵敏度氨基酸分析：用少于 100 皮摩尔的肽测定氨基酸组成的方法。
影响因子：
2.9
作者：
Böhlen,P;Schroeder,R
通讯作者：
Schroeder,R
牛 II 型腺苷环状 3,5-磷酸依赖性蛋白激酶催化亚基的氨基酸序列。
影响因子：
2.9
作者：
Shoji,S;Ericsson,LH;Walsh,KA;Fischer,EH;Titani,K
通讯作者：
Titani,K