Antibiotic resistance and clonal diversity of invasive Staphylococcus aureus in the rural Ashanti Region, Ghana.
Antibiotic resistance and clonal diversity of invasive Staphylococcus aureus in the rural Ashanti Region, Ghana.
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DOI：
10.1186/s12879-016-2048-3
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发表时间：
2016-11-29
影响因子：
3.7
通讯作者：
May J
中科院分区：
文献类型：
--
作者：
Dekker D;Wolters M;Mertens E;Boahen KG;Krumkamp R;Eibach D;Schwarz NG;Adu-Sarkodie Y;Rohde H;Christner M;Marks F;Sarpong N;May J
金黄色葡萄球菌是加纳从血液培养中分离出的最常见的病原体之一，但对农村地区血液感染的流行病学描述甚少。本研究的目的是调查沙门氏菌的抗菌药物敏感性和克隆多样性。金黄色葡萄球菌导致加纳阿散蒂地区两家医院的血液感染。入院时对所有发热患者（≥37.5 ℃）进行血培养。抗生素敏感性试验。金黄色葡萄球菌分离株通过VITEK 2系统进行。采用多重聚合酶链反应（PCR）检测沙门氏菌。金黄色葡萄球菌特异性nuc基因、Panton-Valentine杀白细胞素（PVL）和耐甲氧西林S.金黄色葡萄球菌（MRSA）特异性mecA和mecC基因。S.通过SPA分型评估金黄色葡萄球菌。总共培养了9，834份血液样本，其中0.6%（n = 56）为S阳性。金黄色。20例耐多药（MDR）菌株的耐药率为35.7%（n = 20）。金黄色葡萄球菌菌株，其中一种是MRSA。常见抗生素的耐药率最高，包括青霉素（n = 55; 98.2%）、四环素（n = 32; 57.1%）和甲氧苄啶/磺胺甲恶唑（n = 26; 46.4%）。在所有的S。金黄色葡萄球菌PVL基因携带率为75.0%（n = 42）。我们发现了25个不同的温泉类型，其中t355（n = 11; 19.6%），t314（n = 8; 14.3%），t084（n = 8; 14.3%）和t311（n = 5; 8.9%）是主要的。该研究显示出对当地可用抗生素的抗生素耐药性水平高得惊人。遗传多样性和PVL阳性的甲氧西林敏感S。金黄色葡萄球菌（MSSA）是高的，可以代表一个水库的毒力PVL阳性MRSA克隆的出现。
Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S. aureus isolates was carried out by the VITEK 2 system. Multiplex polymerase chain reaction (PCR) was used to detect S. aureus-specific nuc gene, Panton-Valentine leukocidin (PVL), and methicillin-resistant S. aureus (MRSA)-specific mecA and mecC genes. The population structure of S. aureus was assessed by spa typing. In total, 9,834 blood samples were cultured, out of which 0.6% (n = 56) were positive for S. aureus. Multidrug resistance (MDR) was detected in 35.7% (n = 20) of the S. aureus strains, of which one was a MRSA. The highest rate of antibiotic resistance was seen for commonly available antibiotics, including penicillin (n = 55; 98.2%), tetracycline (n = 32; 57.1%) and trimethoprim/sulfamethoxazole (n = 26; 46.4%). Of all S. aureus strains, 75.0% (n = 42) carried the PVL-encoding genes. We found 25 different spa types with t355 (n = 11; 19.6%), t314 (n = 8; 14.3%), t084 (n = 8; 14.3%) and t311 (n = 5; 8.9%) being predominant. The study exhibited an alarmingly large level of antibiotic resistance to locally available antibiotics. The frequency of genetically diverse and PVL-positive methicillin-sensitive S. aureus (MSSA) was high and could represent a reservoir for the emergence of virulent PVL-positive MRSA clones.
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影响因子：
4.2
作者：
Shittu AO;Okon K;Adesida S;Oyedara O;Witte W;Strommenger B;Layer F;Nübel U
通讯作者：
Nübel U
影响因子：
9.4
作者：
McClure, JA;Conly, JM;Zhang, KY
通讯作者：
Zhang, KY
影响因子：
5.2
作者：
Pichon, Bruno;Hill, Robert;Kearns, Angela M.
通讯作者：
Kearns, Angela M.
影响因子：
56.3
作者：
Shallcross, Laura J.;Fragaszy, Ellen;Johnson, Anne M.;Hayward, Andrew C.
通讯作者：
Hayward, Andrew C.
影响因子：
3.7
作者：
Egyir, Beverly;Guardabassi, Luca;Larsen, Anders Rhod
通讯作者：
Larsen, Anders Rhod