Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria.
Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria.
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DOI：
10.1186/1471-2180-11-92
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发表时间：
2011-05-05
期刊：
影响因子：
4.2
通讯作者：
Nübel U
中科院分区：
文献类型：
--
作者：
Shittu AO;Okon K;Adesida S;Oyedara O;Witte W;Strommenger B;Layer F;Nübel U
金黄色葡萄球菌是引起医院和社区广泛感染的重要病原体。为了获得足够的信息以治疗S。金黄色葡萄球菌感染，了解耐药模式的趋势至关重要。此外，还对S.金黄色葡萄球菌、克隆特性及其地理分布对于建立适当的感染控制方案至关重要。在本研究中，68 S.使用表型和分子方法表征2009年1月至4月期间从尼日利亚的临床和非临床来源获得的金黄色葡萄球菌分离株。所有的S。金黄色葡萄球菌分离株对替考拉宁、万古霉素、磷霉素、夫西地酸、利福平、达托霉素、莫匹罗星、利奈唑胺和替加环素敏感。16%的分离株对苯唑西林耐药，而55%和72%的分离株分别对四环素和甲氧苄啶/磺胺甲恶唑（复方新诺明）耐药（表1）。微量肉汤稀释法与多重PCR检测耐药基因具有良好的相关性。金黄色葡萄球菌对红霉素、庆大霉素、甲氧西林和四环素耐药。共鉴定出28种SPA类型，其中甲氧西林敏感株的优势SPA类型为S。金黄色葡萄球菌（MSSA）分离株为t084（13株）。在迈杜古里（尼日利亚东北部），t037-ST 241-SCCmecIII型是唯一鉴定的克隆，而在尼日利亚西南部，观察到MRSA分离株（t451-ST 8-SCCmecV; t008-ST 94-SCCmecIV; t002-ST 5-SCCmecV; t064-ST 8-SCCmecV）之间的多样性。毒素基因seh和etd分别在属于克隆复合物CC 1、CC 80和序列型ST 25的菌株中检测到。PVL阳性菌株在MSSA中的比例较高（40%）。大多数PVL阳性MSSA分离株来自伤口感染，与克隆复合物CC 1、CC 30、CC 121和序列类型ST 152相关。耐药谱的S。表型和分子生物学方法的应用为研究金黄色葡萄球菌的耐药性和分子多样性提供了有用的信息。尼日利亚的金黄色葡萄球菌。在所有卫生机构中检测到的属于各种克隆复合体的PVL阳性MSSA分离株的高比例是一个主要问题，既是严重感染的来源，也是可能导致PVL阳性MRSA出现的潜在储存库。这项研究首次提供了关于沙门氏菌抗生素耐药基因性质的基线信息。金黄色葡萄球菌分离株在尼日利亚。有必要在尼日利亚卫生保健机构中减少MRSA和PVL阳性MSSA克隆的传播和建立。
Staphylococcus aureus is an important pathogen causing a wide range of infections in the hospital and community setting. In order to have adequate information for treatment of S. aureus infections, it is crucial to understand the trends in the antibiotic-resistance patterns. In addition, the occurrence and changes in types of S. aureus, clonal identities, and their geographic spread is essential for the establishment of adequate infection control programmes. In this study, 68 S. aureus isolates obtained from clinical and non-clinical sources in Nigeria between January and April 2009 were characterized using phenotypic and molecular methods. All the S. aureus isolates were susceptible to teicoplanin, vancomycin, phosphomycin, fusidic acid, rifampicin, daptomycin, mupirocin, linezolid and tigecycline. Sixteen percent of the isolates were resistant to oxacillin, while 55% and 72% of isolates were resistant to tetracycline and trimethoprim/sulphamethoxazole (cotrimoxazole), respectively (Table 1). There was excellent correlation between the broth microdilution assay and detection of antibiotic resistance genes by the multiplex PCR, in the determination of S. aureus resistance to erythromycin, gentamicin, methicillin and tetracycline. A total of 28 spa types were identified in the study, and the predominant spa type among the methicillin-susceptible S. aureus (MSSA) isolates was t084 (13 isolates). The t037-ST241-SCCmecIII type was the only clone identified in Maiduguri (North-East Nigeria) while in South-West Nigeria, diversity among the MRSA isolates (t451-ST8-SCCmecV; t008-ST94-SCCmecIV; t002-ST5-SCCmecV; t064-ST8-SCCmecV) was observed. The toxin genes seh and etd were detected in isolates affiliated with clonal complexes CC1, CC80 and sequence type ST25, respectively. The proportion of PVL-positive isolates among MSSA was high (40%). Most of the PVL-positive MSSA isolates were obtained from wound infections and associated with clonal complexes CC1, CC30, CC121 and with sequence type ST152. Antibiotic resistance profile of S. aureus (MSSA and MRSA) from Nigeria The use of phenotypic and molecular methods provided useful information on antibiotic resistance and molecular diversity of S. aureus in Nigeria. The high proportion of PVL-positive MSSA isolates affiliated to various clonal complexes and detected in all the health institutions is a major concern, both as a source of severe infections and as a potential reservoir that could lead to the emergence of PVL-positive MRSA. This study presents the first baseline information on the nature of the antibiotic resistance genes from S. aureus isolates in Nigeria. There is the need to curtail the spread and establishment of MRSA and PVL-positive MSSA clones in Nigerian health care institutions.
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影响因子：
14.2
作者：
Baranovich, T.;Zaraket, H.;Suzuki, H.
通讯作者：
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影响因子：
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