Biocompatibility of synthetic poly(ester urethane)/polyhedral oligomeric silsesquioxane matrices with embryonic stem cell proliferation and differentiation.
Biocompatibility of synthetic poly(ester urethane)/polyhedral oligomeric silsesquioxane matrices with embryonic stem cell proliferation and differentiation.
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DOI：
10.1002/term.272
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发表时间：
2010-10
影响因子：
3.3
通讯作者：
Otaigbe, Joshua U.
中科院分区：
文献类型：
--
作者：
Guo, Yan-Lin;Wang, Wenshou;Otaigbe, Joshua U.
关键词：
将多面体低聚倍半硅氧烷（POSS）作为结构单元引入到聚（酯氨基甲酸酯）（PEU）中导致具有增加的机械强度和热稳定性的PEU/POSS杂化聚合物。这种新聚合物的一个吸引人的特点是，当以薄膜的形式浇铸时，它形成多孔基质，使其在组织工程中具有潜在的用途。在这项研究中，我们提出了详细的PEU/POSS矩阵的显微镜分析，并证明其与细胞培养的生物相容性。PEU/POSS聚合物形成具有开孔和互连凹槽的连续多孔基质。根据SEM图像分析，计算出每mm 2基质面积约有950个孔径为1 - 15 μm的孔。孔占据的面积约占基质面积的7.6%。使用小鼠胚胎干细胞（ESC），我们证明了PEU/POSS矩阵提供了良好的支持细胞增殖和分化。在优化以维持自我更新的细胞培养条件下，在PEU/POSS基质上生长的ESC表现出未分化的形态，表达多能性标志物，并且具有与在明胶上生长的细胞相似的生长速率。当被诱导分化时，ESCs经历了戏剧性的形态学变化，其特征在于克隆形成能力的丧失和细胞大小的增加以及扩展的细胞骨架网络。分化的细胞能够形成紧密包埋在基质上的连续单层。PEU/POSS基质与ESC增殖/分化之间的优异相容性证明了在未来基于ESC的组织工程中使用PEU/POSS聚合物的潜力。
Incorporation of polyhedral oligomeric silsesquioxanes (POSS) into poly (ester urethane)s (PEU) as a building block results in a PEU/POSS hybrid polymer with increased mechanical strength and thermostability. An attractive feature of the new polymer is that it forms a porous matrix when cast in the form of a thin film, making it potentially useful in tissue engineering. In this study, we present detailed microscopic analysis of the PEU/POSS matrix and demonstrate its biocompatibility with cell culture. The PEU/POSS polymer forms a continuous porous matrix with open pores and interconnected grooves. From SEM image analysis, it is calculated that there are about 950 pores per mm2 of the matrix area with pore size ranging from 1 to 15 μm in diameter. The area occupied by the pores represents approximately 7.6 % of matrix area. Using mouse embryonic stem cells (ESCs), we demonstrate that the PEU/POSS matrix provides excellent support for cell proliferation and differentiation. Under the cell culture condition optimized to maintain self-renewal, ESCs grown on a PEU/POSS matrix exhibit undifferentiated morphology, express pluripotency markers, and have similar growth rate to cells grown on gelatin. When induced for differentiation, ESCs underwent dramatic morphological change, characterized by the loss of clonogenecity and increased cell size with well-expanded cytoskeleton networks. Differentiated cells are able to form a continuous monolayer that is closely embedded on the matrix. The excellent compatibility between the PEU/POSS matrix and ESC proliferation/differentiation demonstrates the potential of using PEU/POSS polymers in future ESC-based tissue engineering.
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影响因子：
2.9
作者：
Chakraborty, Samujjwal;Kang, Baobin;Guo, Yan-Lin
通讯作者：
Guo, Yan-Lin
DOI：
10.1023/a:1024951330265
发表时间：
2003-08-01
影响因子：
3.7
作者：
Brodbeck, WG;Colton, E;Anderson, JM
通讯作者：
Anderson, JM
影响因子：
4.6
作者：
Wang, Wenshou;Guo, Yanlin;Otaigbe, Joshua U.
通讯作者：
Otaigbe, Joshua U.
影响因子：
56.9
作者：
LANGER, R;VACANTI, JP
通讯作者：
VACANTI, JP
影响因子：
5.5
作者：
Nanda, Ajaya K.;Wicks, Douglas A.;Otaigbe, Joshua U.
通讯作者：
Otaigbe, Joshua U.