Basic biological characterization of feline morbillivirus.

Basic biological characterization of feline morbillivirus.
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DOI:
10.1292/jvms.14-0623
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发表时间:
2015-05
期刊:
The Journal of veterinary medical science
影响因子:
--
通讯作者:
Miyazawa T
Miyazawa T
中科院分区:
其他
文献类型:
--
作者:
Koide R;Sakaguchi S;Miyazawa T

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猫麻疹病毒(FmoPV)是最近在患有慢性肾炎的家猫身上发现的一种新病毒。尽管FmoPV在慢性肾炎中具有潜在的作用,但人们对其生物学特性知之甚少。在本研究中,我们利用间接免疫荧光技术建立了FmoPV的定量检测方法。在一周内检测FmoPV的病毒滴度。用以前用于病毒分离的聚丙稀®或胰酶处理并不能增加病毒滴度。FmoPV在4°C下非常稳定,保持高滴度至少12天。60℃和70℃热处理分别在10min和2min内有效地灭活了FmoPV。本文报道的FmoPV的生物学特性将有助于建立有效的病毒分离方法,并将为采取措施降低FmoPV感染风险提供重要信息。
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Treatment with polybrene® or trypsin which was previously used in virus isolation did not augment the virus titers. FmoPV was notably stable at 4°C, retaining high titers for at least 12 days. Heat-treatment at 60°C and 70°C effectively inactivated FmoPV in 10 and 2 min, respectively. The biological characteristics of FmoPV reported here will be beneficial for establishing an efficient virus isolation method and will provide important information to take a measure to reduce the risk of FmoPV infection.
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发表时间: 2012-04-03
影响因子: 11.1
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