Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.
Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.
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DOI：
10.1038/nature14064
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发表时间：
2015-01-22
期刊：
影响因子：
64.8
通讯作者：
Johannes, Ludger
中科院分区：
文献类型：
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作者：
Renard, Henri-Francois;Simunovic, Mijo;Lemiere, Joel;Boucrot, Emmanuel;Garcia-Castillo, Maria Daniela;Arumugam, Senthil;Chambon, Valerie;Lamaze, Christophe;Wunder, Christian;Kenworthy, Anne K.;Schmidt, Anne A.;McMahon, Harvey T.;Sykes, Cecile;Bassereau, Patricia;Johannes, Ludger
在胞吞作用期间，能量被投入以使含货物的质膜内陷的颈部变窄至相对区段自发聚结的半径，从而导致通过胞吞摄取载体的断裂而分离。在网格蛋白途径中，发动蛋白使用来自GTP水解的机械能来达到此效果，并由BIN/两性物理素/Rvs（BAR）结构域的含有蛋白质内亲和素辅助。不依赖网格蛋白的内吞事件通常较少依赖于发动蛋白，并且在这些情况下，BAR结构域蛋白（如内亲和素）是否有助于断裂仍然未被探索。在这里，我们发现，endoA 2（endoA 2）的具体和功能与非常早期的摄取结构，诱导的细菌滋贺和霍乱毒素，这两者都是网格蛋白独立的内吞货物。在受控的体外系统中，endoA 2在断裂前重塑膜。此外，我们证明，endoA 2，发动蛋白，肌动蛋白有助于平行的滋贺毒素诱导的小管的切断。我们的研究结果建立了一个新的功能endoA 2网格蛋白独立的内吞作用。他们记录了不同的断裂因子以添加剂的方式起作用，并预测特定摄取过程中的特异性来自通用模块的定义组合。我们的研究结果最终突出了以前未被注意的endoA 2和拉力驱动的动态断裂之间的联系。
During endocytosis, energy is invested to narrow the necks of cargo-containing plasma membrane invaginations to radii at which the opposing segments spontaneously coalesce, thereby leading to the detachment by scission of endocytic uptake carriers. In the clathrin pathway, dynamin uses mechanical energy from GTP hydrolysis to this effect, assisted by the BIN/amphiphysin/Rvs (BAR) domain-containing protein endophilin. Clathrin-independent endocytic events are often less reliant on dynamin, and whether in these cases BAR domain proteins such as endophilin contribute to scission has remained unexplored. Here we found that endophilin-A2 (endoA2) specifically and functionally associates with very early uptake structures that are induced by the bacterial Shiga and cholera toxins, which both are clathrin-independent endocytic cargoes. In controlled in vitro systems, endoA2 reshapes membranes prior to scission. Furthermore, we demonstrate that endoA2, dynamin, and actin contribute in parallel to the scission of Shiga toxin-induced tubules. Our results establish a novel function of endoA2 in clathrin-independent endocytosis. They document that distinct scission factors operate in an additive manner, and predict that specificity within a given uptake process arises from defined combinations of universal modules. Our findings finally highlight a previously unnoticed link between membrane scaffolding by endoA2 and pulling force-driven dynamic scission.
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影响因子：
16.2
作者：
Milosevic I;Giovedi S;Lou X;Raimondi A;Collesi C;Shen H;Paradise S;O'Toole E;Ferguson S;Cremona O;De Camilli P
通讯作者：
De Camilli P
影响因子：
11.4
作者：
Nesterov, A;Carter, RE;Sorkin, A
通讯作者：
Sorkin, A
影响因子：
14.9
作者：
Jordan, M;Schallhorn, A;Wurm, FM
通讯作者：
Wurm, FM
DOI：
10.1074/jbc.m112.444869
发表时间：
2013-03-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Meinecke M;Boucrot E;Camdere G;Hon WC;Mittal R;McMahon HT
通讯作者：
McMahon HT
语法16和语法5是有效地逆行几种外源和内源性货物蛋白的逆行运输所必需的。
影响因子：
4
作者：
通讯作者：
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