Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate.
Halotolerant microbial consortia able to degrade highly recalcitrant plant biomass substrate.
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DOI：
10.1007/s00253-017-8714-6
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发表时间：
2018-03
影响因子：
5
通讯作者：
Falcao Salles J
中科院分区：
文献类型：
--
作者：
Cortes-Tolalpa L;Norder J;van Elsas JD;Falcao Salles J
微生物在盐度胁迫下对植物源化合物的降解仍未得到充分的研究。为了提高木质纤维素单体的产量，通常需要对木质纤维素材料进行预处理，但预处理会导致高含盐量，产生一个盐水环境，从而引起影响后续下游工艺的技术考虑。在这里，我们通过在顽固的碳和能量来源（即麦秸）上富集盐沼土壤微生物群，构建了耐盐木质纤维素降解微生物群落。在新鲜底物（适应阶段）上生长6个周期，然后在预消化（高度顽固）底物（稳定阶段）上生长4个周期，获得了菌落。数据表明，典型的耐盐细菌占选定菌群的很大一部分。这些“训练”在新鲜底物上逐渐表现更好，但当使用高度顽固的底物时观察到转变。群落中优势菌群为滨海绿丝霉、北黄杆菌、大褐藻、恶臭假单胞菌和子午线盐单胞菌。有趣的是，真菌很少存在，并且受到基质组成变化的负面影响。在适应阶段结束时，窄纹索弧菌是唯一恢复的真菌菌株，而由于顽固底物的存在，它被取消了选择。选择后一种底物的菌落比选择新鲜底物的菌落表现出更高的纤维素和木质素降解，表明在转化底物的顽固性区域方面存在专业化。此外，我们的研究结果表明，与真菌相比，细菌在盐水条件下降解顽固性木质纤维素方面起着主要作用。最后的联合体构成了一个有趣的木质纤维素水解卤化酶的来源，可以用来提高降解过程的效率，同时降低相关的成本。本文的在线版本（10.1007/s00253-017-8714-6）包含补充资料，仅供授权用户使用。
The microbial degradation of plant-derived compounds under salinity stress remains largely underexplored. The pretreatment of lignocellulose material, which is often needed to improve the production of lignocellulose monomers, leads to high salt levels, generating a saline environment that raises technical considerations that influence subsequent downstream processes. Here, we constructed halotolerant lignocellulose degrading microbial consortia by enriching a salt marsh soil microbiome on a recalcitrant carbon and energy source, i.e., wheat straw. The consortia were obtained after six cycles of growth on fresh substrate (adaptation phase), which was followed by four cycles on pre-digested (highly-recalcitrant) substrate (stabilization phase). The data indicated that typical salt-tolerant bacteria made up a large part of the selected consortia. These were “trained” to progressively perform better on fresh substrate, but a shift was observed when highly recalcitrant substrate was used. The most dominant bacteria in the consortia were Joostella marina, Flavobacterium beibuense, Algoriphagus ratkowskyi, Pseudomonas putida, and Halomonas meridiana. Interestingly, fungi were sparsely present and negatively affected by the change in the substrate composition. Sarocladium strictum was the single fungal strain recovered at the end of the adaptation phase, whereas it was deselected by the presence of recalcitrant substrate. Consortia selected in the latter substrate presented higher cellulose and lignin degradation than consortia selected on fresh substrate, indicating a specialization in transforming the recalcitrant regions of the substrate. Moreover, our results indicate that bacteria have a prime role in the degradation of recalcitrant lignocellulose under saline conditions, as compared to fungi. The final consortia constitute an interesting source of lignocellulolytic haloenzymes that can be used to increase the efficiency of the degradation process, while decreasing the associated costs. The online version of this article (10.1007/s00253-017-8714-6) contains supplementary material, which is available to authorized users.
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影响因子：
11.4
作者：
Kinet, R.;Destain, J.;Delvigne, F.
通讯作者：
Delvigne, F.
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
5
作者：
Jimenez, Diego Javier;Korenblum, Elisa;van Elsas, Jan Dirk
通讯作者：
van Elsas, Jan Dirk
影响因子：
3
作者：
Okeke, Benedict C.;Lu, Jue
通讯作者：
Lu, Jue
影响因子：
11.4
作者：
Goldbeck, Rosana;Ramos, Mayla M.;Maugeri-Filho, Francisco
通讯作者：
Maugeri-Filho, Francisco