Direct observation of microtubule dynamics in Reticulomyxa: Unusually rapid length changes and microtubule sliding
Direct observation of microtubule dynamics in Reticulomyxa: Unusually rapid length changes and microtubule sliding
复制标题
直接观察网状黏菌中的微管动力学：异常快速的长度变化和微管滑动
DOI：
10.1002/cm.970170308
复制
发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
M. Schliwa
中科院分区：
文献类型：
--
作者：
Yih;M. Schliwa
Microtubule dynamics has been studied extensively in vitro, but comparatively little information is available on the in vivo behavior of microtubules. Here we report on the assembly, disassembly, and sliding of microtubules in the giant freshwater amoeba, Reticulomyxa. We have found that treating the cell with 0.25% trypsin induces the rapid formation of exceedingly flat areas within the reticulopodial network, allowing for the direct observation of microtubule behavior by DIC optics and computer-enhanced video microscopy. In flattened areas, microtubule sliding occurs at rates of between 1 and 6.5 μm/sec. The average rate of microtubule assembly is 1.6 μm/sec, while microtubule disassembly takes place at about 4 μm/sec and can reach up to 19.5 μm/sec. We also observed many cases where a microtubule forms a hairpin loop and eventually breaks, resulting in bidirectional disassembly from the point of breakage. Our observations demonstrate sliding of cytoplasmic microtubules in vivo. The high rates of microtubule assembly/disassembly in this cell type are difficult to reconcile with conventional views of association and dissociation processes at microtubule ends and suggest unconventional mechanisms for the growth and shrinkage of microtubules.
异体中天然存在的含微管蛋白的副晶体：免疫细胞化学鉴定和功能意义。
影响因子：
--
作者：
Rupp,G;Bowser,SS;Mannella,CA;Rieder,CL
通讯作者：
Rieder,CL
微管和人造膜之间的动态相互作用。
影响因子：
2.9
作者：
Caron,JM;Berlin,RD
通讯作者：
Berlin,RD
影响因子：
--
作者：
FISHER, GW;CONRAD, PA;TAYLOR, DL
通讯作者：
TAYLOR, DL