Biocontrol of the root-knot nematode Meloidogyne incognita by a nematicidal bacterium Pseudomonas simiae MB751 with cyclic dipeptide.
Biocontrol of the root-knot nematode Meloidogyne incognita by a nematicidal bacterium Pseudomonas simiae MB751 with cyclic dipeptide.
复制标题
通过具有环状二肽的杀线虫细菌假单胞菌 MB751 对根结线虫南方根结线虫的生物防治。
DOI：
10.1002/ps.6470
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发表时间：
2021-05
影响因子：
4.1
通讯作者：
Li Lin
中科院分区：
文献类型：
--
作者：
Sun Xiaowen;Zhang Run;Ding Meijuan;Liu Yongxuan;Li Lin
背景
BACKGROUND
Root-knot nematodes (RKN) are harmful plant-parasitic nematodes that cause serious damage to plant hosts. In the long-term practice of RKN management, bacterial nematicides have attracted increasing attention as an effective biocontrol means. Here we determined the active substances against Meloidogyne incognita from a nematicidal bacterium, developed a biocontrol agent (BCA) based on optimized culture processes. The effects of the BCA on RKN control and plant growth-promoting were evaluated in tomato pot trials.
RESULTS
Pseudomonas simiae strain MB751 exhibiting significant nematicidal activity against M. incognita second-stage juveniles (J2) with approximately 80% mortality (with culture supernatant, 96% volume percentage) was isolated from a vineyard. A set of purification and identification experiments was performed to determine the main nematicidal component in MB751. A cyclic dipeptide Cyclo(L-Pro-L-Leu) was identified with a lethal concentration necessary to kill 50% of the population (LC50 ) of 65.3 μg mL-1 against M. incognita J2. Following optimization trials on culture medium/fermentation conditions, such as the single factor test, Plackett-Burman test, steepest ascent, and response surface methodology experiments, the MB751 fermentation broth was then prepared as a BCA via a cold-air drying process. The BCA and was evaluated in tomato pot experiments for effectiveness in suppressing M. incognita. Significant effects on M. incognita suppression and plant-growth promotion as well as induced systemic resistance to M. incognita of tomato, were observed.
CONCLUSION
The cyclic dipeptide-producing bacterium P. simiae MB751 exhibited high nematicidal activity and performance. Further development of this BCA should be pursued for the management of M. incognita in agriculture.
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影响因子：
4.1
作者：
Verinder Wahla;D. K. Maheshwari;V. Bajpai
通讯作者：
Verinder Wahla;D. K. Maheshwari;V. Bajpai
影响因子：
6.9
作者：
Desrut, Antoine;Moumen, Bouziane;Vriet, Cecile
通讯作者：
Vriet, Cecile
影响因子：
4.1
作者：
Kumari, Sarita;Vaishnav, Anukool;Choudhary, Devendra Kumar
通讯作者：
Choudhary, Devendra Kumar
影响因子：
0.7
作者：
A. Sikandar;M. Zhang;Y. Y. Wang-Y.;X. F. Zhu;X. Liu;H. Fan;Y. Xuan;L. Chen;Y. Duan
通讯作者：
A. Sikandar;M. Zhang;Y. Y. Wang-Y.;X. F. Zhu;X. Liu;H. Fan;Y. Xuan;L. Chen;Y. Duan
影响因子：
4.8
作者：
M.A. Radwan;S. Farrag;M. M. Abu-Elamayem-M.;N. Ahmed
通讯作者：
M.A. Radwan;S. Farrag;M. M. Abu-Elamayem-M.;N. Ahmed