Imaging cellular distribution of fluorescent supramolecular nanofibers

Imaging cellular distribution of fluorescent supramolecular nanofibers
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荧光超分子纳米纤维的细胞分布成像

DOI:
10.1007/s11426-015-5521-8
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发表时间:
2016-06
影响因子:
9.6
通讯作者:
Yang Zhimou
Yang Zhimou
中科院分区:
化学1区
文献类型:
--
作者:
Wang Jingyu;Zheng Jing;Cai Yanbin;Zheng Jinxin;Gao Jie;Gong Qingqiu;Yang Zhimou

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在本研究中,我们使用4-硝基-2,1,3-苯并恶二唑(NBD)作为肽的芳香封端基团来构建超分子纳米纤维。利用NBD的荧光特性,我们可以直接观察自组装纳米纤维的细胞分布。我们发现NBD-FFETIGGY纳米纤维在四种哺乳动物细胞和两种植物细胞中的分布是不同的。纳米纤维主要分布在两种哺乳动物细胞和一种植物细胞的表面,而在其他细胞的胞内空间。纳米纤维的不同分布导致两种不同细胞系中纳米纤维的不同蛋白质结合模式。我们相信,已经为纳米纤维的细胞分布成像提供了一个有用且通用的平台,这可以为自组装纳米结构的生物功能提供有用的信息。
In this study, we used the 4-nitro-2,1,3-benzoxadiazole (NBD) as an aromatic capping group for a peptide to construct the supramolecular nanofibers. Taking the advantage of the fluorescence property of NBD, we could directly observe the cellular distribution of the self-assembled nanofibers. We found that the distributions of the nanofibers of NBD-FFETIGGY are different in four mammalian cells and two plant cells. The nanofibers are mainly located at the surface of two mammalian cells and one plant cell, while in the intracellular space of other cells. Different distributions of nanofibers lead to different protein binding patterns of the nanofibers in two different cell lines. We believe that a useful and versatile platform has been offered to the image cellular distribution of nanofibers, which can provide useful information to the biological functions of the self-assembled nanostructures.
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