The independent acquisition of plant root nitrogen-fixing symbiosis in Fabids recruited the same genetic pathway for nodule organogenesis.
The independent acquisition of plant root nitrogen-fixing symbiosis in Fabids recruited the same genetic pathway for nodule organogenesis.
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DOI：
10.1371/journal.pone.0064515
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gherbi H
中科院分区：
文献类型：
--
作者：
Svistoonoff S;Benabdoun FM;Nambiar-Veetil M;Imanishi L;Vaissayre V;Cesari S;Diagne N;Hocher V;de Billy F;Bonneau J;Wall L;Ykhlef N;Rosenberg C;Bogusz D;Franche C;Gherbi H
只有隶属于被子植物4纲10科的Fabid支系的物种才能与土壤细菌形成固氮根瘤共生体。这涉及豆科植物(豆科)和副豆科植物(大麻科)与革兰氏阴性变态细菌有关，统称为根瘤菌，以及放线菌植物与革兰氏阳性放线菌属有关。钙和钙调蛋白依赖的蛋白激酶(CCaMK)是导致根瘤和丛枝菌根共生(AM)的共同信号通路的关键组成部分，在根瘤器官发生和侵染过程之间的交叉信号中发挥着核心作用。在这里，我们表明CCaMK也是成功的放线菌形成和与AM真菌相互作用所必需的，也能够恢复紫花苜蓿突变体的结瘤和AM共生。此外，我们还在两个放线菌物种中表达了缺乏自抑制/CaM结构域的自活性CgCCaMK：木麻黄(木麻黄科)和铁皮盘藻(鼠李科)，前者具有细胞内感染途径，后者以祖先细胞间感染机制为特征。在这两个物种中，我们发现在根瘤菌-豆科共生中独立于Frankia的结瘤诱导类似于对CCaMK激活的反应，并得出结论：无论感染模式如何，放线菌器官发生的调控都是保守的。有人认为，根瘤菌和放线菌的共生起源于一个共同的祖先，具有几个独立的进化起源。我们的发现与控制根瘤菌和Frankia根瘤器官发生的相似遗传途径的招募是一致的。
Only species belonging to the Fabid clade, limited to four classes and ten families of Angiosperms, are able to form nitrogen-fixing root nodule symbioses (RNS) with soil bacteria. This concerns plants of the legume family (Fabaceae) and Parasponia (Cannabaceae) associated with the Gram-negative proteobacteria collectively called rhizobia and actinorhizal plants associated with the Gram-positive actinomycetes of the genus Frankia. Calcium and calmodulin-dependent protein kinase (CCaMK) is a key component of the common signaling pathway leading to both rhizobial and arbuscular mycorrhizal symbioses (AM) and plays a central role in cross-signaling between root nodule organogenesis and infection processes. Here, we show that CCaMK is also needed for successful actinorhiza formation and interaction with AM fungi in the actinorhizal tree Casuarina glauca and is also able to restore both nodulation and AM symbioses in a Medicago truncatula ccamk mutant. Besides, we expressed auto-active CgCCaMK lacking the auto-inhibitory/CaM domain in two actinorhizal species: C. glauca (Casuarinaceae), which develops an intracellular infection pathway, and Discaria trinervis (Rhamnaceae) which is characterized by an ancestral intercellular infection mechanism. In both species, we found induction of nodulation independent of Frankia similar to response to the activation of CCaMK in the rhizobia-legume symbiosis and conclude that the regulation of actinorhiza organogenesis is conserved regardless of the infection mode. It has been suggested that rhizobial and actinorhizal symbioses originated from a common ancestor with several independent evolutionary origins. Our findings are consistent with the recruitment of a similar genetic pathway governing rhizobial and Frankia nodule organogenesis.
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影响因子：
3.5
作者：
Imanishi, Leandro;Vayssieres, Alice;Svistoonoff, Sergio
通讯作者：
Svistoonoff, Sergio
影响因子：
3.5
作者：
Laplaze, L;Duhoux, E;Pawlowski, K
通讯作者：
Pawlowski, K
影响因子：
4.9
作者：
Banba, Mari;Gutjahr, Caroline;Imaizumi-Anraku, Haruko
通讯作者：
Imaizumi-Anraku, Haruko
影响因子：
3.5
作者：
DIOUF, D;GHERBI, H;BOGUSZ, D
通讯作者：
BOGUSZ, D
DOI：
10.1111/j.1365-313x.2010.04228.x
发表时间：
2010-07-01
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Hayashi T;Banba M;Shimoda Y;Kouchi H;Hayashi M;Imaizumi-Anraku H
通讯作者：
Imaizumi-Anraku H