Quantitative proteomic analysis of brain in rats with intrauterine infection
Quantitative proteomic analysis of brain in rats with intrauterine infection
复制标题
宫内感染大鼠脑部蛋白质组定量分析
DOI：
--
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hong Cui
中科院分区：
文献类型：
--
作者：
Lijun Yang;Hong Cui
宫内感染/炎症可显著影响围产期脑发育，并导致脑结构和功能的显著改变。然而，子宫内感染的病理生理机制仍然难以捉摸，治疗仍然有限。蛋白质组学分析可能有助于深入了解潜在的机制，从而有助于改善临床治疗。本研究将SD孕鼠随机分为脂多糖（LPS）组和对照组，从孕18天开始注射LPS或生理盐水，共2天。使用无标记鸟枪分析法对两组中4天新生大鼠幼仔的脑组织进行定量分析。结果显示，对照组共鉴定出283个蛋白，宫内感染组共鉴定出260个蛋白。与对照组相比，LPS组有11个蛋白表达上调2倍，11个蛋白表达下调2倍。经大卫数据库分析发现，差异表达蛋白包括线粒体组分或蛋白质、细胞骨架和细胞器等;分子功能分析表明，大部分蛋白质参与离子结合、核苷酸结合和酶结合等;生物学过程分析表明，大部分蛋白质参与神经系统发育的调控、离子转运的调控、蛋白质复合体的生物合成和细胞骨架的组装等方面进行了研究，并利用STRING 9.0软件对这些蛋白质之间存在的复杂相互作用进行了分析和探讨。最后，我们用Western blot对随机选取的蛋白质进行了倍数变化的验证，发现与蛋白质组学的结果有相同的趋势。本研究对未成年大鼠宫内感染的蛋白质组学研究进行了全面的筛选。
Intrauterine infection/inflammation can significantly affect perinatal brain development and result in significant alterations in brain structure and function. However, the pathophysiological mechanism of intrauterine infection remains elusive and therapy remains limited. Proteomics analysis might provide insight into potential mechanism and thus help to improve the clinical treatment. In this study, pregnant SD rats were divided into lipopolysaccharide (LPS) group and control group randomly, LPS or saline was injected for two days from gestation 18. Quantitative analysis of the brain tissue of 4 day-neonatal rat pups in two groups was performed using label-free shotgun analysis. Results showed that there were 283 proteins identified from control group and 260 proteins from intrauterine infection group. 11 proteins were 2 folds up-regulated and 11 proteins were 2 folds down-regulated in LPS group compared to control group. After analysis using DAVID database, we found that the differentially expressed proteins comprised mitochondrial component or proteins, cytoskeleton and organelle et al. Molecular function analysis revealed that the majority of proteins were involved in ion binding, nucleotide binding and enzyme binding et al. Biological process analysis showed that the majority of proteins were involved in regulation of nervous system development, regulation of ion transport, protein complex biogenesis and cytoskeleton organization et al. We then used STRING 9.0 software to analyze and explore the complex interactions existed among these proteins. Finally, we used Western blot to verify the fold changes of some proteins that we randomly chose and found that there existed the same trend compared to the results of proteomics. This novel study performed a full-scale screening of the proteomics research in intrauterine infection of immature rat.
登录
查看更多内容
影响因子：
7.2
作者：
O'Callaghan, Michael E.;MacLennan, Alastair H.;Dekker, Gustaaf A.
通讯作者：
Dekker, Gustaaf A.
影响因子：
3.7
作者：
Leghmar K;Cenac N;Rolland M;Martin H;Rauwel B;Bertrand-Michel J;Le Faouder P;Bénard M;Casper C;Davrinche C;Fournier T;Chavanas S
通讯作者：
Chavanas S
影响因子：
1.7
作者：
Fukuda, Sumio;Yokoi, Kyoko;Goto, Haruo
通讯作者：
Goto, Haruo
DOI：
10.1097/01.aoa.0000389603.63510.ef
发表时间：
2010-12
期刊：
Obstetric Anesthesia Digest
影响因子：
--
作者：
J. Been;I. Rours;R. Kornelisse;V. L. Passos;B. Kramer;T. A. Schneider;R. R. D. Krijger-R.;L. Zimmermann
通讯作者：
J. Been;I. Rours;R. Kornelisse;V. L. Passos;B. Kramer;T. A. Schneider;R. R. D. Krijger-R.;L. Zimmermann
影响因子：
11.8
作者：
Boppana, Suresh B.;Ross, Shannon A.;Fowler, Karen B.
通讯作者：
Fowler, Karen B.