Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies.
Moving towards improved surveillance and earlier diagnosis of aquatic pathogens: From traditional methods to emerging technologies.
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DOI：
10.1111/raq.12674
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发表时间：
2022-09
影响因子：
10.4
通讯作者：
Cable, Joanne
中科院分区：
文献类型：
--
作者：
MacAulay, Scott;Ellison, Amy R.;Kille, Peter;Cable, Joanne
关键词：
早期和准确的诊断以及有效的监测是减轻传染病影响的关键。然而，由于隐藏的生物多样性和物质限制，这在水生环境中尤其具有挑战性。传统的诊断学，如视觉诊断和组织病理学，仍然被广泛使用，但越来越多的技术进步，如便携式下一代测序(NGS)和人工智能(AI)，正在被测试用于早期诊断。基于视觉诊断的最直接的方法依赖于专业知识和经验，但为监视提供了基础。未来的计算遥感方法，如人工智能图像诊断和无人机监视，最终将在不影响灵敏度的情况下降低劳动力成本，但它们需要资本和基础设施投资。在过去的30年里，分子技术发展迅速，从标准的 ，到环介导的等温扩增(LAMP)，再到NGS方法，提供了一系列支持当前流行的EDNA诊断的技术。在人类诊断学的发展推动下，现在有巨大的变革潜力。在这里，我们将当前的监测和诊断技术与那些可以用于或开发用于水环境的技术进行比较，对照高灵敏度、特异性、快速诊断和成本效益这三个黄金标准理想。
Early and accurate diagnosis is key to mitigating the impact of infectious diseases, along with efficient surveillance. This however is particularly challenging in aquatic environments due to hidden biodiversity and physical constraints. Traditional diagnostics, such as visual diagnosis and histopathology, are still widely used, but increasingly technological advances such as portable next generation sequencing (NGS) and artificial intelligence (AI) are being tested for early diagnosis. The most straightforward methodologies, based on visual diagnosis, rely on specialist knowledge and experience but provide a foundation for surveillance. Future computational remote sensing methods, such as AI image diagnosis and drone surveillance, will ultimately reduce labour costs whilst not compromising on sensitivity, but they require capital and infrastructural investment. Molecular techniques have advanced rapidly in the last 30 years, from standard PCR through loop‐mediated isothermal amplification (LAMP) to NGS approaches, providing a range of technologies that support the currently popular eDNA diagnosis. There is now vast potential for transformative change driven by developments in human diagnostics. Here we compare current surveillance and diagnostic technologies with those that could be used or developed for use in the aquatic environment, against three gold standard ideals of high sensitivity, specificity, rapid diagnosis, and cost‐effectiveness.
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EDNA水溶液的年度时间序列分析揭示了湖泊生态系统生物多样性的生态相关动力学。
影响因子：
16.6
作者：
Bista I;Carvalho GR;Walsh K;Seymour M;Hajibabaei M;Lallias D;Christmas M;Creer S
通讯作者：
Creer S
DOI：
10.5539/gjhs.v8n3p72
发表时间：
2015-06-25
期刊：
Global journal of health science
影响因子：
--
作者：
Alturkistani HA;Tashkandi FM;Mohammedsaleh ZM
通讯作者：
Mohammedsaleh ZM
影响因子：
3.1
作者：
Caipang, CMA;Haraguchi, I;Aoki, T
通讯作者：
Aoki, T
影响因子：
5
作者：
Albetis, Johanna;Duthoit, Sylvie;Dedieu, Gerard
通讯作者：
Dedieu, Gerard
影响因子：
4.5
作者：
Bastos Gomes, Giana;Hutson, Kate S.;Jerry, Dean R.
通讯作者：
Jerry, Dean R.