Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid.
Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid.
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肠毒素大肠杆菌质粒的核苷酸序列分析。
DOI：
10.1093/dnares/dsp015
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Tsuji T
中科院分区：
文献类型：
--
作者：
Ochi S;Shimizu T;Ohtani K;Ichinose Y;Arimitsu H;Tsukamoto K;Kato M;Tsuji T
我们在这里报告的完整核苷酸序列pEntH 10407（65 - 147 bp），肠毒素大肠杆菌肠毒素质粒（Ent质粒），这是自我传播的低频率。在质粒中，我们鉴定了100个可编码多肽的开放阅读框（ORF）。这些ORF包括编码热不稳定（LT）和热稳定（STIa）肠毒素的区域，编码质粒复制工具的区域和不完全的tra（接合）区域。LT和STIa区域相距13.5 kb，被三个IS 1和一个IS 600以相反的阅读方向包围，表明肠毒素基因可能已水平转移到质粒中。我们确定了一个单一的RepFIIA复制区（2.0 kb），包括RepA蛋白类似于RepA 1，RepA 2，RepA 3和RepA 4。不完整的tra区由17个tra基因组成，它们与R100的相应基因几乎相同，并显示ISEc 8和ISEc 8-like元件的多个插入的证据。这些数据表明，pEntH 10407具有细菌毒力质粒的嵌合性质特征，其包含关于其进化的信息。尽管tra基因最初可能使pEntH 10407具有与R100相同程度的自转移性，但在pEntH 10407的tra区域中发生了多个插入事件，使其移动的较少。另一个自传递质粒可能有助于pEntH 10407有效地转化H10407菌株。在本文中，我们提出了另一种可能性：肠道致病性H10407菌株可能是通过使用不完整的tra区域以低速率自动转移pEntH 10407而形成的。
We report here the complete nucleotide sequence of pEntH10407 (65 147 bp), an enterotoxigenic Escherichia coli enterotoxin plasmid (Ent plasmid), which is self-transmissible at low frequency. Within the plasmid, we identified 100 open reading frames (ORFs) which could encode polypeptides. These ORFs included regions encoding heat-labile (LT) and heat-stable (STIa) enterotoxins, regions encoding tools for plasmid replication and an incomplete tra (conjugation) region. The LT and STIa region was located 13.5 kb apart and was surrounded by three IS1s and an IS600 in opposite reading orientations, indicating that the enterotoxin genes may have been horizontally transferred into the plasmid. We identified a single RepFIIA replication region (2.0 kb) including RepA proteins similar to RepA1, RepA2, RepA3 and RepA4. The incomplete tra region was made up of 17 tra genes, which were nearly identical to the corresponding genes of R100, and showed evidence of multiple insertions of ISEc8 and ISEc8-like elements. These data suggest that pEntH10407 has the mosaic nature characteristic of bacterial virulence plasmids, which contains information about its evolution. Although the tra genes might originally have rendered pEntH10407 self-transferable to the same degree as R100, multiple insertion events have occurred in the tra region of pEntH10407 to make it less mobile. Another self-transmissible plasmid might help pEntH10407 to transfer efficiently into H10407 strain. In this paper, we suggest another possibility: that the enterotoxigenic H10407 strain might be formed by auto-transfer of pEntH10407 at a low rate using the incomplete tra region.
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影响因子：
2.6
作者：
Jiang, Y;Yang, F;Jin, Q
通讯作者：
Jin, Q
影响因子：
3.1
作者：
EVANS, DG;SILVER, RP;GORBACH, SL
通讯作者：
GORBACH, SL
影响因子：
15.9
作者：
Gaastra, W;Svennerholm, AM
通讯作者：
Svennerholm, AM
影响因子：
11.4
作者：
GERDES, K;BECH, FW;VONMEYENBURG, K
通讯作者：
VONMEYENBURG, K
影响因子：
3.2
作者：
SMITH, HR;WILLSHAW, GA;ROWE, B
通讯作者：
ROWE, B