Use of ELISpot assay to study HBs-specific B cell responses in vaccinated and HBV infected humans.
Use of ELISpot assay to study HBs-specific B cell responses in vaccinated and HBV infected humans.
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使用 ELISpot 测定研究接种疫苗和 HBV 感染人群中 HBs 特异性 B 细胞反应
DOI：
10.1038/s41426-018-0034-0
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发表时间：
2018-02-16
影响因子：
13.2
通讯作者：
Wu C
中科院分区：
文献类型：
--
作者：
Tian C;Chen Y;Liu Y;Wang S;Li Y;Wang G;Xia J;Zhao XA;Huang R;Lu S;Wu C
乙型肝炎表面抗体（HBsAb）在预防乙型肝炎病毒（HBV）感染中起着至关重要的作用，文献对其进行了广泛研究。与此同时，在接种者和HBV感染者中乙型肝炎表面抗原（HBs）特异性B细胞的状况得到的关注有限。在目前的研究中，我们采用了高度特异性的B细胞酶联免疫点（ELISpot）检测来分析各种临床环境中的乙型肝炎特异性B细胞：在接受额外HBV疫苗增强之前和之后有HBV疫苗接种史的健康个体，处于不同临床阶段的慢性HBV感染者，接受或不接受特定类型的抗病毒治疗，或是否接受了一剂HBV疫苗。在所有这些病例中，B细胞ELISpot法被有效地用于枚举hbs特异性B细胞的频率。虽然当前报告的重点是建立这种检测方法在HBV研究中的实用性，但在这项基于HBV特异性B细胞在各种条件下的概况和动态的初步研究中，进行了许多有趣的观察。这些信息有助于指导未来设计针对慢性乙型肝炎的新治疗策略。
Hepatitis B surface antibody (HBsAb) plays a critical role in protecting against infection of hepatitis B virus (HBV) and were extensively studied in literature. At the same time, the status of hepatitis B surface antigen (HBs)-specific B cells in both vaccinated and HBV infected people received limited attention. In the current study, we adopted a highly specific B-cell Enzyme Linked ImmunoSpot (ELISpot) assay to analyze HBs-specific B cells in various clinical settings: healthy individuals with the history of HBV vaccination before and after receiving an extra HBV vaccine boost, people chronically infected with HBV (CHB) in various clinical stages, with or without a particular type anti-viral treatment, or whether receiving a dose of HBV vaccine. In all of these cases, B-cell ELISpot assay was used effectively in enumerating the frequency of HBs-specific B cells. While the focus of the current report was to establish the utility of this assay for HBV research, a number of interesting observations were made in this pilot study based on the profiles and dynamics of HBs-specific B cells in various conditions. Such information is useful to guide the future work in designing novel therapeutic strategies against CHB.
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影响因子：
7.3
作者：
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通讯作者：
Fink K
影响因子：
2.2
作者：
CZERKINSKY, CC;NILSSON, LA;TARKOWSKI, A
通讯作者：
TARKOWSKI, A
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作者：
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作者：
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通讯作者：
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