Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.
Key features of the two-intron Saccharomyces cerevisiae gene SUS1 contribute to its alternative splicing.
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DOI：
10.1093/nar/gkr497
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发表时间：
2011-10
影响因子：
14.9
通讯作者：
Johnson TL
中科院分区：
文献类型：
--
作者：
Hossain MA;Rodriguez CM;Johnson TL
选择性前mRNA剪接允许真核蛋白质组的急剧扩增，并促进细胞对环境条件变化的反应。酿酒酵母基因SUS 1编码一种参与mRNA输出和组蛋白H2 B去泛素化的蛋白质，含有两个内含子;第一个内含子中的非规范序列有助于其保留，这是植物和真菌中常见的选择性剪接形式。在这里，我们表明，SUS 1剪接模式的变化响应于环境的变化，如温度升高，和保留的内含子产品是无义介导的衰变。不同剪接因子的活性决定SUS 1剪接的模式，包括内含子保留和外显子跳跃。出乎意料的是，3′内含子的去除受到上游内含子剪接的影响，这表明跨外显子相互作用影响内含子的去除。不同SUS 1亚型的产生对细胞功能很重要，因为我们发现在sus 1 Δ细胞中观察到的温度敏感性和组蛋白H2 B去泛素化缺陷仅被SUS 1 cDNA部分抑制，但能够进行剪接的SUS 1补充了这些表型。这些数据说明了S的作用。酵母可变剪接在组蛋白修饰和细胞功能中的作用，揭示了含有多个内含子的酵母基因剪接的重要机制。
Alternative pre-mRNA splicing allows dramatic expansion of the eukaryotic proteome and facilitates cellular response to changes in environmental conditions. The Saccharomyces cerevisiae gene SUS1, which encodes a protein involved in mRNA export and histone H2B deubiquitination, contains two introns; non-canonical sequences in the first intron contribute to its retention, a common form of alternative splicing in plants and fungi. Here we show that the pattern of SUS1 splicing changes in response to environmental change such as temperature elevation, and the retained intron product is subject to nonsense-mediated decay. The activities of different splicing factors determine the pattern of SUS1 splicing, including intron retention and exon skipping. Unexpectedly, removal of the 3′ intron is affected by splicing of the upstream intron, suggesting that cross-exon interactions influence intron removal. Production of different SUS1 isoforms is important for cellular function, as we find that the temperature sensitivity and histone H2B deubiquitination defects observed in sus1Δ cells are only partially suppressed by SUS1 cDNA, but SUS1 that is able to undergo splicing complements these phenotypes. These data illustrate a role for S. cerevisiae alternative splicing in histone modification and cellular function and reveal important mechanisms for splicing of yeast genes containing multiple introns.
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影响因子：
4.5
作者：
Gunderson FQ;Johnson TL
通讯作者：
Johnson TL
影响因子：
4.5
作者：
Preker, PJ;Kim, KS;Guthrie, C
通讯作者：
Guthrie, C
DOI：
10.1093/protein/5.8.827
发表时间：
1992-12-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
PRODROMOU, C;PEARL, LH
通讯作者：
PEARL, LH
影响因子：
16
作者：
Dong, Shuyun;Li, Chunfang;He, Feng
通讯作者：
He, Feng
DOI：
10.1007/978-0-387-77374-2_8
发表时间：
2007-01-01
期刊：
ALTERNATIVE SPLICING IN THE POSTGENOMIC ERA
影响因子：
--
作者：
Martinez-Contreras, Rebeca;Cloutier, Philippe;Chabot, Benoit
通讯作者：
Chabot, Benoit