Low power gas discharge plasma mediated inactivation and removal of biofilms formed on biomaterials.
Low power gas discharge plasma mediated inactivation and removal of biofilms formed on biomaterials.
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DOI：
10.1016/j.cap.2012.12.024
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发表时间：
2013-03-20
影响因子：
2.4
通讯作者：
Liang, Jun F.
中科院分区：
文献类型：
--
作者：
Traba, Christian;Chen, Long;Liang, Jun F.
气体放电等离子体的抗菌活性已经研究了一段时间。然而，血浆的高生物膜灭活活性只是最近才报道的。研究表明，与产生的等离子体相关的蚀刻效应代表了不期望的效应，其可能导致活细菌重新定位，从而导致污染扩散。同时，这些高功率等离子体的强蚀刻效应也可能改变生物材料的表面化学性质，影响生物相容性。在这项研究中，我们研究了低功率气体放电等离子体生物膜灭活和去除的效率和效果。在氧气、氮气和氩气这三种气体中，放电氧气由于其对生物膜中细菌的良好杀灭能力和温和的刻蚀作用而表现出最好的抗生物膜活性。低功率放电氧气可完全杀灭并去除附着表面的死菌，但对材料的生物相容性影响不大。去除生物膜后留在再生表面上的DNA对组织细胞生长没有任何负面影响。相反，发现这些细胞接种在再生表面上的生长显著增加。这些结果表明，低功率放电氧气在生物材料的生物膜处理和留置器械的去污方面具有潜在的应用前景。
The antibacterial activity of gas discharge plasma has been studied for quiet some time. However, high biofilm inactivation activity of plasma was only recently reported. Studies indicate that the etching effect associated with plasmas generated represent an undesired effect, which may cause live bacteria relocation and thus contamination spreading. Meanwhile, the strong etching effects from these high power plasmas may also alter the surface chemistry and affect the biocompatibility of biomaterials. In this study, we examined the efficiency and effectiveness of low power gas discharge plasma for biofilm inactivation and removal. Among the three tested gases, oxygen, nitrogen, and argon, discharge oxygen demonstrated the best anti-biofilm activity because of its excellent ability in killing bacteria in biofilms and mild etching effects. Low power discharge oxygen completely killed and then removed the dead bacteria from attached surface but had negligible effects on the biocompatibility of materials. DNA left on the regenerated surface after removal of biofilms did not have any negative impact on tissue cell growth. On the contrary, dramatically increased growth was found for these cells seeded on regenerated surfaces. These results demonstrate the potential applications of low power discharge oxygen in biofilm treatments of biomaterials and indwelling device decontaminations.
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影响因子：
2.8
作者：
Joaquin, Jonathan C.;Kwan, Calvin;Brelles-Marino, Graciela
通讯作者：
Brelles-Marino, Graciela
影响因子：
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作者：
Traba C;Liang JF
通讯作者：
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作者：
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通讯作者：
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