Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.
Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.
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DOI：
10.1371/journal.pone.0052846
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nolan DP
中科院分区：
文献类型：
--
作者：
Woods K;Nic a'Bhaird N;Dooley C;Perez-Morga D;Nolan DP
鞭毛附着是非洲锥虫的一个明显的形态特征，但人们对分子水平上附着的要求知之甚少。这项研究描述了一种以前未描述过的膜蛋白 FLA3，它在布氏锥虫鞭毛附着中发挥着重要作用。 FLA3 高度 N-糖基化，位于鞭毛附着区，似乎是血流阶段特异性蛋白质。 FLA3 mRNA 的消融迅速导致鞭毛脱离，并伴随细长血流形式的胞质分裂失败，但对前循环形式没有影响。在 dsRNA 诱导后不久，鞭毛脱离就很明显，并且新合成的鞭毛在从鞭毛袋中出现后通常完全脱离。 12 小时内，大多数细胞在现有的附着鞭毛旁边具有分离的鞭毛。这些结果表明，参与附着的蛋白质在新旧附着区之间不共享。在其他方面，分离的鞭毛看起来正常，尽管失去了方向性运动，但它们跳动很快，并且它们具有明显正常的轴丝和鞭毛旁杆结构。当 FLA3 耗尽时，鞭毛附着区似乎被破坏。因此，虽然鞭毛附着是锥虫生命周期的一个组成特征，但附着需要蛋白质水平上的阶段特异性元件。
Flagellar attachment is a visibly striking morphological feature of African trypanosomes but little is known about the requirements for attachment at a molecular level. This study characterizes a previously undescribed membrane protein, FLA3, which plays an essential role in flagellar attachment in Trypanosoma brucei. FLA3 is heavily N-glycosylated, locates to the flagellar attachment zone and appears to be a bloodstream stage specific protein. Ablation of the FLA3 mRNA rapidly led to flagellar detachment and a concomitant failure of cytokinesis in the long slender bloodstream form but had no effect on the procyclic form. Flagellar detachment was obvious shortly after induction of the dsRNA and the newly synthesized flagellum was often completely detached after it emerged from the flagellar pocket. Within 12 h most cells possessed detached flagella alongside the existing attached flagellum. These results suggest that proteins involved in attachment are not shared between the new and old attachment zones. In other respects the detached flagella appear normal, they beat rapidly although directional motion was lost, and they possess an apparently normal axoneme and paraflagellar rod structure. The flagellar attachment zone appeared to be disrupted when FLA3 was depleted. Thus, while flagellar attachment is a constitutive feature of the life cycle of trypanosomes, attachment requires stage specific elements at the protein level.
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DOI：
10.1074/jbc.m111.244772
发表时间：
2011-07-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Graham SA;Antonopoulos A;Hitchen PG;Haslam SM;Dell A;Drickamer K;Taylor ME
通讯作者：
Taylor ME
影响因子：
4.8
作者：
Rubotham, J;Woods, K;Nolan, DP
通讯作者：
Nolan, DP
影响因子：
2.1
作者：
RUIZMORENO, L;BIJOVSKY, AT;COLLI, W
通讯作者：
COLLI, W
影响因子：
11.4
作者：
Kohl, L;Robinson, D;Bastin, P
通讯作者：
Bastin, P
DOI：
10.1098/rstb.1989.0037
发表时间：
1989-06-12
影响因子：
6.3
作者：
SHERWIN, T;GULL, K
通讯作者：
GULL, K