Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV.
Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV.
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氨基肽酶N是肠道病毒冠状病毒TGEV的主要受体。
DOI：
10.1038/357417a0
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发表时间：
1992-06-04
期刊：
影响因子：
64.8
通讯作者：
Laude H
中科院分区：
文献类型：
--
作者：
Delmas B;Gelfi J;L'Haridon R;Vogel LK;Sjöström H;Norén O;Laude H
像许多动物病毒一样，冠状病毒的特征是受限的宿主范围和组织对流1。可传播的胃炎病毒（TGEV）是一种主要的病原体，导致新生儿致命的腹泻，在覆盖小肠小绒毛的分化肠细胞中有选择地复制。为了研究感染的分子决定因素，我们表征了该病毒用于结合和进入宿主细胞的表面分子。在这里，我们报告说，氨基肽酶N是一种在肠上皮细胞的顶部膜上表达的过多酶，用作TGEV的受体。选择单克隆抗体，以通过猪细胞系的TGEV阻断感染的能力。他们认识到MR 150K的刷膜膜蛋白，通过弹药末端测序被鉴定为氨基肽酶N。两条证据支持了肽酶本身充当受体的观点。首先，与纯化为均匀性的Aminopep-tidase N的病毒体。其次，氨基肽酶N的重组表达通过TGEV赋予了其他非疗程细胞系的感染性。
CORONAVIRUSES, like many animal viruses, are characterized by a restricted host range and tissue tropism1. Transmissible gastroenteritis virus (TGEV), a major pathogen causing a fatal diarrhoea in newborn pig, replicates selectively in the differentiated enterocytes covering the villi of the small intestine2. To investigate the molecular determinants of the infection, we characterized the surface molecule used by the virus for binding and entry into host cells. Here we report that aminopeptidase N, an ectoenzyme abundantly expressed at the apical membrane of the enterocytes, serves as a receptor for TGEV. Monoclonal antibodies were selected for their ability to block infection by TGEV of porcine cell lines. They recognized a brush-border membrane protein of Mr, 150K, which was identified as aminopeptidase N by ammo-terminal sequencing. Two lines of evidence supported the view that the peptidase itself acts as a receptor. First, virions bound specifically to aminopep-tidase N that was purified to homogeneity. Second, recombinant expression of aminopeptidase N conferred infectivity by TGEV to an otherwise non-permissive cell line.
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影响因子：
7.8
作者：
GORVEL, JP;MISHAL, Z;MAROUX, S
通讯作者：
MAROUX, S
影响因子：
64.5
作者：
MADDON, PJ;DALGLEISH, AG;AXEL, R
通讯作者：
AXEL, R
影响因子：
64.5
作者：
MENDELSOHN, CL;WIMMER, E;RACANIELLO, VR
通讯作者：
RACANIELLO, VR
DOI：
10.1111/j.1432-1033.1978.tb12476.x
发表时间：
1978-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
SJOSTROM, H;NOREN, O;CHRISTIANSEN, L
通讯作者：
CHRISTIANSEN, L
影响因子：
3.5
作者：
OLSEN, J;COWELL, GM;NOREN, O
通讯作者：
NOREN, O