Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show sodium channel accumulations.
Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show sodium channel accumulations.
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DOI：
10.1186/1471-2202-13-29
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发表时间：
2012-03-19
期刊：
影响因子：
2.4
通讯作者：
Levinson SR
中科院分区：
文献类型：
--
作者：
Henry MA;Luo S;Levinson SR
牙髓是疼痛的常见来源，用于研究外周炎性疼痛机制。结果表明，大多数纤维是无髓鞘的，但最近的研究结果表明，在实验动物的许多牙髓传入纤维起源于髓鞘在更近端的位置。在这里，我们使用人类牙髓和共聚焦显微镜检查染色的神经丝重（NFH），一种蛋白质通常在有髓传入，与其他标志物的关系，以测试的可能性，无髓牙髓传入来源于有髓轴突。研究的其他染色关系包括髓鞘碱性蛋白（MBP）、识别所有神经纤维的蛋白基因产物（PGP）9.5、识别交感神经纤维的酪氨酸羟化酶（TH）、识别结节部位的接触素相关蛋白（aspr）、识别施万细胞和钠通道（NaChs）的S-100。结果显示NFH表达在大多数PGP9.5纤维中，除了具有TH的纤维，并且包括NFH在缺乏MBP的轴突中的广泛表达。具有NFH和MBP的纤维如预期的那样在结节部位显示NaCh簇，但令人惊讶的是，NaCh积聚也见于具有NFH的无髓鞘纤维和缺乏雪旺细胞缔合的具有NFH的纤维中。NFH在大多数轴突中的表达表明许多牙髓传入的有髓起源，而无髓纤维中NaCh簇的存在表明有髓纤维的无髓段形成NaCh积累的固有能力。这些发现对使用牙髓研究疼痛机制具有广泛的影响，并提出了可能的新机制，负责氯化钠簇的形成和神经元的兴奋性。
The dental pulp is a common source of pain and is used to study peripheral inflammatory pain mechanisms. Results show most fibers are unmyelinated, yet recent findings in experimental animals suggest many pulpal afferents originate from fibers that are myelinated at more proximal locations. Here we use the human dental pulp and confocal microscopy to examine the staining relationships of neurofilament heavy (NFH), a protein commonly expressed in myelinated afferents, with other markers to test the possibility that unmyelinated pulpal afferents originate from myelinated axons. Other staining relationships studied included myelin basic protein (MBP), protein gene product (PGP) 9.5 to identify all nerve fibers, tyrosine hydroxylase (TH) to identify sympathetic fibers, contactin-associated protein (caspr) to identify nodal sites, S-100 to identify Schwann cells and sodium channels (NaChs). Results show NFH expression in most PGP9.5 fibers except those with TH and include the broad expression of NFH in axons lacking MBP. Fibers with NFH and MBP show NaCh clusters at nodal sites as expected, but surprisingly, NaCh accumulations are also seen in unmyelinated fibers with NFH, and in fibers with NFH that lack Schwann cell associations. The expression of NFH in most axons suggests a myelinated origin for many pulpal afferents, while the presence of NaCh clusters in unmyelinated fibers suggests an inherent capacity for the unmyelinated segments of myelinated fibers to form NaCh accumulations. These findings have broad implications on the use of dental pulp to study pain mechanisms and suggest possible novel mechanisms responsible for NaCh cluster formation and neuronal excitability.
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DOI：
10.1023/a:1007053411667
发表时间：
1999-04-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Ching, W;Zanazzi, G;Salzer, JL
通讯作者：
Salzer, JL
影响因子：
3.7
作者：
Bax DA;Little SE;Gaspar N;Perryman L;Marshall L;Viana-Pereira M;Jones TA;Williams RD;Grigoriadis A;Vassal G;Workman P;Sheer D;Reis RM;Pearson AD;Hargrave D;Jones C
通讯作者：
Jones C
影响因子：
2.3
作者：
Hass, Nicole;Schwarzenbacher, Karin;Breer, Heinz
通讯作者：
Breer, Heinz
影响因子：
7.4
作者：
Henry, Michael A.;Freking, Angelique R.;Levinson, S. Rock
通讯作者：
Levinson, S. Rock
影响因子：
16.2
作者：
Eshed, Y;Feinberg, K;Peles, E
通讯作者：
Peles, E