Injectable Acylhydrazone-Linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation.
Injectable Acylhydrazone-Linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation.
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用于持续蛋白质释放和细胞封装的可注射酰腙连接 RAFT 聚合物水凝胶。
DOI：
10.1002/adhm.202101284
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发表时间：
2022-04
影响因子：
10
通讯作者：
Lin CC
中科院分区：
文献类型：
--
作者：
Lin FY;Dimmitt NH;Moraes de Lima Perini M;Li J;Lin CC
采用可逆加成-断裂链转移(RAFT)聚合方法合成了一类新型温度响应性聚合物PADO。由二丙酮丙烯酰胺(DAAM)、二乙二醇乙醚丙烯酸酯(DEGA)和低聚乙二醇甲基醚丙烯酸酯(OEGA)共聚合成的PADO聚合物相在高于其LCST(36°C~42°C)的温度下，由于乙二醇短侧链之间疏水作用的增强而分离。PADO聚合物溶液具有可注射的剪切变稀性能，并在37℃时迅速达到溶胶-凝胶转变(<；5min)。当己二酸二肼(ADH)将DAAM上的酮部分连接起来时，PADO聚合物形成了交联的可注射的酰肼水凝胶，由于pH敏感的酰肼键，该水凝胶可在温和的酸性环境中降解。PH响应降解动力学可以通过调节聚合物含量和酮/肼的比例来控制。重要的是，可注射的PADO水凝胶具有高度的细胞相容性，可以很容易地配制成pH响应型持续蛋白质输送。采用RAFT聚合法合成了一类新型的含有二丙酮丙烯酰胺(DAAM)、二乙二醇甲基醚丙烯酸酯(DEGA)和低聚乙二醇甲基醚丙烯酸酯(OEGA)的PADO聚合物。通过酰肼化学，PADO聚合物被交联成pH/温度敏感的可注射水凝胶，以实现可调和持续的蛋白质输送。
A new class of temperature responsive polymer, termed PADO, is synthesized by reversible addition-fragmentation chain-transfer (RAFT) polymerization. Synthesized from copolymerization of diacetone acrylamide (DAAM), di(ethylene glycol) ethyl ether acrylate (DEGA), and oligo(ethylene glycol) methyl ether acrylate (OEGA), PADO polymer phase separates at temperature above its LCST (36°C to 42°C) due to enhanced hydrophobic interactions between the short ethylene glycol side chains. Solution of PADO polymers exhibited injectable shear-thinning properties and reached sol-gel transition rapidly (< 5 min) at 37°C. When the ketone moieties on DAAM were linked by adipic acid dihydrazdie (ADH), PADO polymers formed crosslinked and injectable acylhydrazone hydrogels, which were hydrolytically degradable at a mild acidic environment owing to the pH sensitive acylhydrazone bonds. The pH-responsive degradation kinetics could be controlled by tuning polymer contents and ketone/hydrazide ratio. Importantly, the injectable PADO hydrogels were highly cytocompatible and could be easily formulated for pH-responsive sustained protein delivery. A novel class of PADO polymers containing diacetone acrylamide (DAAM), di(ethylene glycol) ethyl ether acrylate (DEGA), and oligo(ethylene glycol) methyl ether acrylate (OEGA) are polymerized via RAFT polymerization. Via acylhydrazone chemistry, the PADO polymers are crosslinked into pH/thermo-sensitive injectable hydrogel for tunable and sustained protein delivery.
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影响因子：
62.1
作者：
Kamaly N;Yameen B;Wu J;Farokhzad OC
通讯作者：
Farokhzad OC
影响因子：
15
作者：
Lutz, Jean-Francois;Akdemir, Oezguer;Hoth, Ann
通讯作者：
Hoth, Ann
影响因子：
5
作者：
Tatara, Yuji;Ohishi, Mitsuru;Rakugi, Hiromi
通讯作者：
Rakugi, Hiromi
影响因子：
5.5
作者：
Pertici, Vincent;Trimaille, Thomas;Gigmes, Didier
通讯作者：
Gigmes, Didier
影响因子：
6
作者：
Kou, Longfa;Xiao, Shuyi;Chen, Ruijie
通讯作者：
Chen, Ruijie