DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
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DOI：
10.1002/anie.201304038
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发表时间：
2013-09-27
影响因子：
16.6
通讯作者：
Davis, Benjamin G.
中科院分区：
文献类型：
--
作者：
Lercher, Lukas;McGouran, Joanna F.;Kessler, Benedikt M.;Schofield, Christopher J.;Davis, Benjamin G.
DNA作为遗传信息的载体，其研究是现代生物学的基石。由互补性决定的DNA杂交使许多基于DNA的功能结构的设计成为可能这些人造系统包括行走器、DNAzymes、[3]和逻辑门虽然典型核碱基的结构支架对于许多目的来说是足够的，但通常需要额外的功能。开发具有新颖电子、[5]荧光、[6]和金属络合[7]性质的改性构件是一个活跃的研究领域。[8]已经开发了多种将具有所需性质的修饰构建块掺入寡脱氧核苷酸（odn）的方法。通常，这些方法采用“线性组装”方法，即在低聚化之前的早期阶段安装改性单体。最常见的是通过固相合成（SPS）将所需要的改性碱掺入odn中这就需要合成合适的新型磷酰胺基块，它们通常是易氧化的在合成过程中所需修饰的稳定性是先决条件。即使满足了这些要求，庞大的构建模块的加入也会降低耦合效率
The study of DNA, as the carrier of genetic information, is a cornerstone of modern biology. DNA hybridization determined by complementarity has allowed the design of many DNA-based functional structures.[1] Such man-made systems include walkers,[2] DNAzymes,[3] and logic gates.[4] While the structural scaffold of canonical nucleobases is sufficient for many purposes, additional functionality is often desirable. The development of modified building blocks with novel electronic,[5] fluorogenic,[6] and metal-complexing [7] properties is an active research area.[8]Multiple methods for the incorporation of modified building blocks with desired properties into oligodeoxynucleotides (ODNs) have been developed. Typically, these employ a “linear assembly” approach where a modified monomer is installed at an early stage, prior to oligomerization. Most common is the incorporation of the desired modified base into ODNs through solid-phase synthesis (SPS).[9] This necessitates the synthesis of appropriate novel phosphoramidite building blocks, which are typically oxidation prone.[10] Stability of the desired modification during the synthesis is a prerequisite. Even when these requirements are met, the incorporation of bulky building blocks can decrease the coupling efficiency.[11]
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