Perfusion and Inflation of the Mouse Lung for Tumor Histology.
Perfusion and Inflation of the Mouse Lung for Tumor Histology.
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DOI：
10.3791/60605
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发表时间：
2020-08-06
期刊：
影响因子：
--
通讯作者：
Edmonds MD
中科院分区：
文献类型：
--
作者：
Davenport ML;Sherrill TP;Blackwell TS;Edmonds MD
评估肺组织学的能力对于肺癌研究和癌症转移领域至关重要。同样重要的是，在不牺牲所采购组织质量的情况下，快速有效地对研究进行尸检。因此，该协议的目标是提出一种快速灌注、充气和固定小鼠肺部以进行下游组织学分析的方法。该方法不标准化肺充气，因此不需要任何特殊程序或设备，而是简单地在通过心脏灌注后直接通过气管滴注固定剂。这可以充分估计肿瘤大小、组织学和评分。该方法还允许在肺组织固定之前收集冷冻组织。该方法的局限性在于它不允许随后对肺进行形态测量量化；然而，对于基因工程小鼠模型 (GEMM)、同基因模型以及异种移植肿瘤和转移研究的肺肿瘤分析来说，它已经足够了。该方法的目的是提出一种简单有效的小鼠肺灌注、充气和固定方法，用于检查肺肿瘤病理学和评估肺转移。
The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to rapidly and efficiently perform necropsies from studies without sacrificing the quality of tissues procured. The goal of this protocol is thus to present a method to rapidly perfuse, inflate, and fix mouse lungs for downstream histological analysis. This method does not standardize lung inflation thus not requiring any special procedures or equipment and instead simply instilling fixative directly through the trachea following perfusion through the heart. This allows for sufficient estimation of tumor size, histology, and scoring. This method also allows for the collection of frozen tissue prior to lung tissue fixation. This method is limited in that it does not allow for later morphometric quantification of the lung; however, it is more than sufficient for lung tumor analysis from genetically engineered mouse models (GEMMs), syngeneic models, as well as xenograft tumor and metastasis studies. The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.
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影响因子：
15.9
作者：
Edmonds, Mick D.;Boyd, Kelli L.;Eischen, Christine M.
通讯作者：
Eischen, Christine M.
影响因子：
1.5
作者：
Renne, R;Fouillet, X;Copley, M
通讯作者：
Copley, M
影响因子：
3.7
作者：
Zhao K;Wei L;Hui H;Dai Q;You QD;Guo QL;Lu N
通讯作者：
Lu N
影响因子：
3.3
作者：
OLDMIXON, EH;SUZUKI, S;HOPPIN, FG
通讯作者：
HOPPIN, FG
影响因子：
3.3
作者：
Vasilescu, Dragos M.;Knudsen, Lars;Hoffman, Eric A.
通讯作者：
Hoffman, Eric A.