A short 5'-flanking region mediates glucose repression of amylase gene expression in Drosophila melanogaster.
A short 5'-flanking region mediates glucose repression of amylase gene expression in Drosophila melanogaster.
复制标题
短的 5 侧翼区域介导果蝇中淀粉酶基因表达的葡萄糖抑制。
作者：
C. Magoulas;L. Bally;A. Loverre;B. Benkel;D. Hickey
α -淀粉酶基因的表达在黑腹果蝇幼虫中受到膳食葡萄糖的高度抑制。在这里，我们发现葡萄糖抑制是由位于转录区上游的DNA序列控制的。将淀粉酶启动子序列与Adh基因转录区融合的重组基因构建在转基因果蝇幼虫中表达。重组基因ADH的表达受到葡萄糖抑制。通过缺失分析和位点定向诱变，以及转化幼虫的表达分析，研究了葡萄糖响应上游区域潜在的顺式作用元件的功能。上游缺失分析表明，淀粉酶基因的总体活性和葡萄糖抑制的必需元件位于转录起始位点上游109-bp的区域。这些上游序列的定点突变表明，位于-31位的TATA基序和位于-109位的一个新的36-bp元件是淀粉酶启动子充分发挥活性所必需的。引入的突变都没有导致葡萄糖反应性的丧失。这些结果表明，在果蝇中，葡萄糖抑制是由涉及多个功能冗余DNA元件的转录机制介导的。
Expression of the alpha-amylase gene is highly repressed by dietary glucose in Drosophila melanogaster larvae. Here, we show that glucose repression is controlled by DNA sequences that are located upstream of the transcribed region. Recombinant gene constructions, in which the amylase promoter sequences were fused with the transcribed region of the Adh gene, were expressed in transgenic Drosophila larvae. The expression of ADH from the recombinant gene was shown to be subject to glucose repression. The function of potential regulatory cis-acting elements within the glucose responsive upstream region was examined by deletion analysis and by site-directed mutagenesis, coupled with expression assays in transformed larvae. The upstream deletion analysis showed that essential elements, both for overall activity and for glucose repression of the amylase gene, are located within a 109-bp region upstream of the transcription start site. Site-directed mutagenesis of these upstream sequences showed that the TATA motif, at position -31, and a novel 36-bp element, at position -109, were necessary for full activity of the amylase promoter. None of the introduced mutations resulted in loss of glucose responsiveness. These results indicate that glucose repression, in Drosophila, is mediated by transcriptional mechanisms that involve multiple, functionally redundant DNA elements.
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DOI：
10.1073/pnas.87.16.6238
发表时间：
1990-08
影响因子：
11.1
作者：
M. Weickert;G. Chambliss
通讯作者：
M. Weickert;G. Chambliss
黑腹果蝇α-淀粉酶基因的分子克隆。
影响因子：
3.3
作者：
Gemmill,RM;Levy,JN;Doane,WW
通讯作者：
Doane,WW
使用 P 元件介导的转化来鉴定影响果蝇 Adh 表达的自然发生变异的分子基础。
影响因子：
3.3
作者：
Laurie-Ahlberg,CC;Stam,LF
通讯作者：
Stam,LF
影响因子：
5.6
作者：
NICHOLSON, WL;PARK, YK;CHAMBLISS, GH
通讯作者：
CHAMBLISS, GH
影响因子：
56.9
作者：
XIAO, H;LIS, JT
通讯作者：
LIS, JT