Urea stress is more akin to EGF exposure than to hypertonic stress in renal medullary cells.
Urea stress is more akin to EGF exposure than to hypertonic stress in renal medullary cells.
复制标题
尿素应激更类似于 EGF 暴露，而不是肾髓质细胞的高渗应激。
DOI：
10.1152/ajprenal.00031.2002
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Cohen,DavidM
中科院分区：
文献类型：
--
作者：
Tian,Wei;Cohen,DavidM
Although urea is considered to be a cell stressor even in renal medullary cells perpetually exposed to this solute in vivo by virtue of the renal concentrating mechanism, aspects of urea signaling resemble that of a peptide mitogen. Urea was compared with epidermal growth factor and hypertonic NaCl or hypertonic mannitol using a large-scale expression array-based approach. The expression profile in response to urea stress more closely resembled that of EGF treatment than hypertonic stress, as determined by hierarchical cluster analysis; the effect of urea+NaCl was equidistant from that of either solute applied individually. Among the most highly urea- and hypertonicity-responsive transcripts were genes that had previously been shown to be responsive to these solutes, validating this approach. Increased expression of the activating transcription factor 3 by urea was newly detected via expression array and confirmed via immunoblot analysis. Earlier, we noted an abrogation of tonicity-dependent gene regulation by urea, primarily in a transient transfection-based model (Tian W and Cohen DM.Am J Physiol Renal Physiol280: F904–F912, 2001). Here we applied K-means cluster analysis to demonstrate that the genes most profoundly up- or downregulated by hypertonic stress were partially restored toward basal levels in the presence of urea pretreatment. These global expression data are consistent with our earlier biochemical studies suggesting that urea affords cytoprotection in this context. In the aggregate, these data strongly support the hypothesis that the urea effect in renal medullary cells resembles that of a peptide mitogen in terms of the adaptive program of gene expression and in terms of cytoprotection from hypertonicity.
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DOI：
10.1152/ajprenal.1999.277.2.f176
发表时间：
1999-08
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Xiao-Yan Yang;Zheng Zhang;D. Cohen
通讯作者：
Xiao-Yan Yang;Zheng Zhang;D. Cohen
尿素和氨对正常大鼠肾细胞肾脏生长调节的研究。
影响因子：
2.5
作者：
Logan,JL
通讯作者：
Logan,JL
尿素使 mIMCD3 细胞对热休克诱导的细胞凋亡敏感：氯化钠的保护。
DOI：
--
发表时间：
2001
期刊：
American Journal of Physiology - Cell Physiology
影响因子：
--
作者：
Chantal Colmont;Stéphanie Michelet;Dominique Guivarc'h;Germain Rousselet
通讯作者：
Germain Rousselet
哺乳动物肾髓质细胞中尿素的信号传导和基因调控。
DOI：
--
发表时间：
2001
期刊：
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
影响因子：
--
作者：
W. Tian;D. Cohen
通讯作者：
D. Cohen
渗透应激蛋白 94 (Osp94)
影响因子：
4.8
作者：
R. Kojima;J. Randall;B. Brenner;S. Gullans
通讯作者：
S. Gullans