Restricted expression of N- and R-cadherin on neurites of the developing chicken CNS
Restricted expression of N- and R-cadherin on neurites of the developing chicken CNS
复制标题
N-和R-钙粘蛋白在发育中的鸡中枢神经系统神经突上的限制性表达
DOI：
10.1523/jneurosci.12-09-03525.1992
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发表时间：
1992
影响因子：
4
通讯作者：
M. Takeichi
中科院分区：
文献类型：
--
作者：
C. Redies;H. Inuzuka;M. Takeichi
两种钙粘蛋白（N-钙粘蛋白和 R-钙粘蛋白）的表达在孵化 6-11 天的鸡胚的中枢神经系统中进行定位，重点是感觉和运动纤维系统。在脊髓中，背索位于外侧的纤维表达 N-钙粘蛋白，而位于内侧的纤维则不表达。这两个纤维系统在中枢神经系统内有不同的路线，但联合形成脊髓背根。在后脑中，N-钙粘蛋白由三叉神经降支（一般躯体感觉）束表达，该束与脊髓背索的 N-钙粘蛋白阳性区相邻。 R-钙粘蛋白不由感觉纤维表达，而是由迷走神经和舌咽神经的内脏运动系统表达，这些系统是N-钙粘蛋白阴性的。表达 R-钙粘蛋白的运动神经突在大脑内的路线与表达 N-钙粘蛋白的感觉神经突不同，尽管它们在大脑表面形成迷走神经的共同感觉/运动根。在体外研究了 N-钙粘蛋白通过同质粘附机制为中枢神经系统内感觉轴突迁移提供引导信号的可能性。将表达 N-钙粘蛋白的感觉脊髓神经节的外植体置于 N-钙粘蛋白转染的神经母细胞瘤细胞上，并观察到轴突的生长。结果表明，感觉轴突解束并紧密遵循转染细胞之间的细胞边界，其中表达高水平的 N-钙粘蛋白。这些结果表明，这两种钙粘蛋白，就像免疫球蛋白分子超家族的成员一样，在小鸡大脑发育过程中以拓扑限制的方式表达。他们还表明，神经突表达的 N-钙粘蛋白可能在引导这些神经突沿着表达相同分子的 CNS 路径中发挥作用。
The expression of two cadherins, N- and R-cadherin, was mapped in the CNS of chicken embryos of 6–11 d incubation, focusing on the sensory and motor fiber systems. In the spinal cord, the laterally located fibers of the dorsal funiculus express N-cadherin while the medially located fibers do not. These two fiber systems have a different course within the CNS but associate to form the spinal dorsal roots. In the hindbrain, N-cadherin is expressed by the descending trigeminal (general somatic sensory) tract, which is contiguous with the N- cadherin-positive zone of the dorsal funiculus of the spinal cord. R- cadherin is not expressed by sensory fibers, but is expressed by the visceral motor system of the vagus and glossopharyngeal nerves, which are N-cadherin negative. The motor neurites expressing R-cadherin have a different course within the brain than the sensory neurites expressing N-cadherin, although they form the common sensory/motor roots of the vagus nerve at the surface of the brain. The possibility that N-cadherin provides a guidance cue for sensory axon migration within the CNS by a homophilic adhesion mechanism was investigated in vitro. Explants from sensory spinal ganglia expressing N-cadherin were placed on N-cadherin-transfected neuroblastoma cells, and axon outgrowth was visualized. Results showed that the sensory axons defasciculate and closely follow the cell-cell boundaries between transfected cells where high levels of N-cadherin are expressed. These results show that the two cadherins, like members of the immunoglobulin superfamily of molecules, are expressed in a topographically restricted fashion during chick brain development. They furthermore suggest that N- cadherin expression by neurites may play a role in guiding these neurites along CNS paths that express the same molecule.
DOI：
10.1091/mbc.2.4.261
发表时间：
1991-04-01
期刊：
CELL REGULATION
影响因子：
--
作者：
SUZUKI, S;SANO, K;TANIHARA, H
通讯作者：
TANIHARA, H
影响因子：
2.7
作者：
DRAZBA, J;LEMMON, V
通讯作者：
LEMMON, V