Cyclic AMP-dependent regulation of differentiation of rat C6 glioma cells by panaxydol
Cyclic AMP-dependent regulation of differentiation of rat C6 glioma cells by panaxydol
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人参醇对大鼠 C6 胶质瘤细胞分化的环 AMP 依赖性调节
DOI：
10.1179/174313209x380919
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发表时间：
2009-04
影响因子：
1.9
通讯作者：
中科院分区：
文献类型：
--
作者：
摘要 目的：初步研究表明人参醇对大鼠C6胶质瘤细胞具有生长抑制和诱导分化作用。然而，这种分化背后的分子机制仍然未知。我们试图研究环磷酸腺苷 (cAMP) 在人参醇诱导的细胞分化中的作用。方法：用人参酚和各种特异性抑制剂处理C6细胞，通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基溴化四唑实验和裸鼠同种移植来评估对细胞生长的抑制作用。在相差显微镜下对星形胶质细胞过程进行量化。分别采用Western blot和划痕实验检测胶质纤维酸性蛋白表达和细胞迁移。另外，通过免疫测定法测定细胞内cAMP浓度。结果：Panaxydol 诱导 C6 细胞内 cAMP 浓度升高。人参醇对 C6 细胞的体外和体内生长抑制作用以及诱导分化作用可以被 cAMP 抑制剂 Rp-腺苷 3',5'-环单磷酸硫酸酯抑制，但不能被蛋白激酶 A 或蛋白激酶 C 特异性抑制剂抑制。结论：这些结果表明cAMP依赖性途径可能通过人参醇调节C6神经胶质瘤细胞的细胞增殖、迁移和分化。
Abstract Objectives: Preliminary works have indicated that panaxydol possesses growth inhibition and induces differentiation in rat C6 glioma cells. However, the molecular mechanism underlying this differentiation remains unknown. We sought to investigate the role of cyclic adenosine monophosphate (cAMP) in cellular differentiation induced by panaxydol. Methods: C6 cells were treated with panaxydol and various specific inhibitors, and the inhibition of cell growth was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and homotransplantation in nude mice. Astrocytic processes were quantified under a phase-contrasted microscope. Glial fibrillary acidic protein expression and cell migration were carried out by Western blot and scratch-wound test, respectively. In addition, the intracellular cAMP concentration was measured by immunoassay. Results: Panaxydol induces the elevation of intracellular cAMP concentration in C6 cells. The effects of growth inhibition in vitro and in vivo and induction of differentiation in C6 cells by panaxydol could be inhibited by the cAMP inhibitor, Rp-adenosine 3′,5′-cyclic monophosphothioate, but not by protein kinase A or protein kinase C specific inhibitors. Conclusion: These results suggest that the cAMP-dependent pathway may regulate cellular proliferation, migration and differentiation in C6 glioma cells by panaxydol.
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影响因子：
3.9
作者：
D. Roymans;B. Grobben;P. Claes;H. Slegers
通讯作者：
D. Roymans;B. Grobben;P. Claes;H. Slegers
影响因子：
56.9
作者：
K. Parker;M. Norenberg;A. Vernadakis
通讯作者：
K. Parker;M. Norenberg;A. Vernadakis
影响因子：
4.2
作者：
A. Nishiyama;Min Yu;J. Drazba;V. Tuohy
通讯作者：
A. Nishiyama;Min Yu;J. Drazba;V. Tuohy
DOI：
10.1054/drup.1998.0062
发表时间：
1999-02
期刊：
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy
影响因子：
--
作者：
M. Nagane;H. Huang;W. Cavenee
通讯作者：
M. Nagane;H. Huang;W. Cavenee
影响因子：
11.2
作者：
L. Bello;G. Carrabba;C. Giussani;V. Lucini;F. Cerutti;F. Scaglione;J. Landré;M. Pluderi;G. Tomei;R. Villani;R. Carroll;P. Black;A. Bikfalvi
通讯作者：
L. Bello;G. Carrabba;C. Giussani;V. Lucini;F. Cerutti;F. Scaglione;J. Landré;M. Pluderi;G. Tomei;R. Villani;R. Carroll;P. Black;A. Bikfalvi