Early transcriptional responses of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7.
Early transcriptional responses of internalization defective Brucella abortus mutants in professional phagocytes, RAW 264.7.
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DOI：
10.1186/1471-2164-14-426
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发表时间：
2013-06-27
期刊：
影响因子：
4.4
通讯作者：
Yoo HS
中科院分区：
文献类型：
--
作者：
Cha SB;Lee WJ;Shin MK;Jung MH;Shin SW;Yoo AN;Kim JW;Yoo HS
牛种布氏杆菌是一种细胞内的人畜共患病原体，可引起人的波状热、心内膜炎、关节炎和骨髓炎，以及牛的流产和不育。这种细菌能够在宿主巨噬细胞中入侵和复制，而不是被这种防御机制清除。因此，了解细菌毒力与宿主细胞之间的相互作用对控制布鲁氏菌病具有重要意义。在此之前，我们产生了内化缺陷突变体并分析了包膜蛋白。本研究旨在评估野生型和内化缺陷突变体感染小鼠巨噬细胞RAW 264.7后早期转录反应的变化。野生型和突变体感染的巨噬细胞均显示促炎细胞因子、趋化因子、凋亡和G蛋白偶联受体（Gpr84、Gpr109a和Adora2b）的表达水平增加，而与介导细胞内运输的小GT3相关的基因减少。此外，细胞粘连素1相互作用蛋白（Cytip）和泛素化相关基因（Arrdc 3和Fbxo21）下调，表明该细菌的生存策略。然而，与野生型感染组相比，我们不能检测到突变感染组中的任何显著变化。总之，很难阐明内化缺陷突变体感染后宿主细胞转录的改变。然而，我们发现了几个新的基因变化相关的GPCR系统，泛素蛋白酶体系统，生长停滞和DNA损伤响应B。流产感染这些发现可能有助于更好地了解宿主与病原体相互作用的分子机制，需要进一步研究。
Brucella abortus is an intracellular zoonotic pathogen which causes undulant fever, endocarditis, arthritis and osteomyelitis in human and abortion and infertility in cattle. This bacterium is able to invade and replicate in host macrophage instead of getting removed by this defense mechanism. Therefore, understanding the interaction between virulence of the bacteria and the host cell is important to control brucellosis. Previously, we generated internalization defective mutants and analyzed the envelope proteins. The present study was undertaken to evaluate the changes in early transcriptional responses between wild type and internalization defective mutants infected mouse macrophage, RAW 264.7. Both of the wild type and mutant infected macrophages showed increased expression levels in proinflammatory cytokines, chemokines, apoptosis and G-protein coupled receptors (Gpr84, Gpr109a and Adora2b) while the genes related with small GTPase which mediate intracellular trafficking was decreased. Moreover, cytohesin 1 interacting protein (Cytip) and genes related to ubiquitination (Arrdc3 and Fbxo21) were down-regulated, suggesting the survival strategy of this bacterium. However, we could not detect any significant changes in the mutant infected groups compared to the wild type infected group. In summary, it was very difficult to clarify the alterations in host cellular transcription in response to infection with internalization defective mutants. However, we found several novel gene changes related to the GPCR system, ubiquitin-proteosome system, and growth arrest and DNA damages in response to B. abortus infection. These findings may contribute to a better understanding of the molecular mechanisms underlying host-pathogen interactions and need to be studied further.
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DOI：
10.3181/0904-rm-124
发表时间：
2009-12
期刊：
Experimental biology and medicine (Maywood, N.J.)
影响因子：
--
作者：
Covert J;Mathison AJ;Eskra L;Banai M;Splitter G
通讯作者：
Splitter G
影响因子：
8.6
作者：
de Bagues, MPJ;Dudal, S;Gross, A
通讯作者：
Gross, A
影响因子：
3.4
作者：
Roux, Christelle M.;Rolan, Hortensia G.;Tsolis, Renee M.
通讯作者：
Tsolis, Renee M.
DOI：
10.1084/jem.20030088
发表时间：
2003-08-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Celli J;de Chastellier C;Franchini DM;Pizarro-Cerda J;Moreno E;Gorvel JP
通讯作者：
Gorvel JP
影响因子：
3.1
作者：
Carbonetti NH
通讯作者：
Carbonetti NH