Differences in follicular function of 3-month-old calves and mature cows.
Differences in follicular function of 3-month-old calves and mature cows.
复制标题
3个月大的犊牛和成年奶牛的卵泡功能的差异。
作者：
M. Driancourt;K. Reynaud;J. Smitz
在体外成熟、受精和发育之后，与母牛相比，小牛中发育到囊胚期的受精卵的百分比通常较低。目前尚不清楚这种体外发育能力低是小牛卵母细胞固有的还是由卵泡成熟改变引起的。后一种可能性在本研究中使用两个卵泡功能的标志物进行了探索：体外类固醇合成的完整卵泡和芳香化酶活性的卵泡壁。直径> 9 mm的小牛卵泡产生雌二醇的能力较低（与奶牛相比降低了10倍），尽管卵泡膜细胞的睾酮输出与奶牛中观察到的相似。这一发现与氚水释放法测得的小牛卵泡颗粒细胞芳香化酶活性较低相一致。使用蛋白质印迹法（β-胡萝卜素和激活素、热休克蛋白90、苗勒氏管抑制物质）和测定法（β-胡萝卜素和激活素）评估小牛和奶牛卵泡液之间的定性和定量差异，以确定这种缺陷性芳香酶是否可以通过调节芳香酶的卵泡蛋白（β-胡萝卜素和激活素、热休克蛋白90、苗勒氏管抑制物质）的量的改变产生。卵泡液蛋白质的Western印迹显示，使用抗-α-β-glycobin抗体时有三条主带（59、57和< 30 kDa）和一条次带（34 kDa），而使用抗-β-glycobin抗体时检测到一条18 kDa的带。小牛卵泡液中含有相似量的所有主要α-谷氨酰胺结合蛋白形式（α和β），但缺少34 kDa α-谷氨酰胺结合蛋白形式。母牛和小牛之间的二聚体阿替宾的量相似，但小牛的小卵泡含有更多的激活素。通过蛋白质印迹法检测到70 kDa（苗勒管抑制物质）和90 kDa（热休克蛋白90）的单一条带。小牛卵泡液中缺少苗勒管抑制物质，小牛卵泡液中热休克蛋白90的含量低于奶牛卵泡液。上述差异均不能解释犊牛卵泡芳香化酶的缺陷。对源自大小和卵泡健康状况匹配的小牛或奶牛卵泡的卵泡壁分泌的[35 S]标记蛋白进行二维分离，在主凝胶上观察到151个斑点，其总结了至少存在一次的所有斑点。小牛中有15个斑点，而奶牛中没有。定量差异也检测到三个点含有更多的蛋白质在奶牛比小牛。这些蛋白质中的一些是否可以改变卵泡或卵母细胞的成熟需要进一步研究。
After in vitro maturation, fertilization and development, the percentage of fertilized eggs developing to the blastocyst stage is usually lower in calves compared with cows. It is unknown whether this low ability to develop in vitro is inherent to calf oocytes or is caused by altered follicular maturation. The latter possibility was explored in the present study using two markers of follicle function: in vitro steroidogenesis by intact follicles and aromatase activity of follicular walls. Calf follicles > 9 mm in diameter had a low ability to produce oestradiol (ten times reduction compared with cows) despite a testosterone output by theca cells which was similar to that observed in cows. This finding is in agreement with the low aromatase activity of granulosa cells of calf follicles measured by tritiated water release assay. Qualitative and quantitative differences between calf and cow follicular fluids were assessed using western blotting (inhibin and activin, heat shock protein 90, Müllerian inhibiting substance) and assays (inhibin and activin) to determine whether this defective aromatase could be produced by alterations in the amounts of follicular proteins modulating aromatase (inhibin and activin, heat shock protein 90, Müllerian inhibiting substance). Western blotting of follicular fluid proteins demonstrated three main bands (59, 57 and < 30 kDa) and one minor band (34 kDa) with the anti-alpha inhibin antibody, whereas a single 18 kDa band was detected when an anti-beta inhibin antibody was used. Calf follicular fluid contained similar amounts of all main inhibin forms (alpha and beta) but a 34 kDa alpha inhibin form was missing. The amounts of dimeric inhibin were similar between cows and calves but small follicles from calves contained more activin. Single bands at 70 kDa (Müllerian inhibiting substance) and 90 kDa (heat shock protein 90) were detected by western blotting. Müllerian inhibiting substance was missing from calf follicular fluid and heat shock protein 90 was present in smaller amounts in calf versus cow follicular fluid. None of the above differences could explain the defective aromatase of calf follicles. Two-dimensional separation of the [35S]-labelled proteins secreted by follicular walls originating from calf or cow follicles matched for size and follicle health was performed and 151 spots were observed on the master gel, which summarized all the spots present at least once. Fifteen spots were present in calves and not in cows. Quantitative differences were also detected with three spots containing more proteins in cows than in calves. Whether some of these proteins can alter maturation of follicles or oocytes requires further investigation.
青春期前小牛胚胎发生卵母细胞能力获得的年龄和激素依赖性。
影响因子：
3.6
作者：
Presicce,GA;Jiang,S;Simkin,M;Zhang,L;Looney,CR;Godke,RA;Yang,X
通讯作者：
Yang,X
卵泡闭锁期间不同形式的抑制素含量的变化。
影响因子：
3.6
作者：
Ireland,JL;Good,TE;Knight,PG;Ireland,JJ
通讯作者：
Ireland,JJ
DOI：
10.1016/0006-291x(86)90427-4
发表时间：
1986-05
影响因子：
3.1
作者：
S. Ying;A. Becker;N. Ling;N. Ueno;R. Guillemin
通讯作者：
S. Ying;A. Becker;N. Ling;N. Ueno;R. Guillemin