The Sesuvium portulacastrum Plasma Membrane Na+/H+ Antiporter SpSOS1 Complemented the Salt Sensitivity of Transgenic Arabidopsis sos1 Mutant Plants
The Sesuvium portulacastrum Plasma Membrane Na+/H+ Antiporter SpSOS1 Complemented the Salt Sensitivity of Transgenic Arabidopsis sos1 Mutant Plants
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Sesuvium portulacastrum 质膜 Na /H 逆向转运蛋白 SpSOS1 补充了转基因拟南芥 sos1 突变体植物的盐敏感性
DOI：
10.1007/s11105-018-1099-6
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发表时间：
2018-06
影响因子：
2.1
通讯作者：
Jiang Xingyu
中科院分区：
文献类型：
--
作者：
Zhou Yang;Yin Xiaochang;Wan Shumin;Hu Yanping;Xie Qing;Li Ruimei;Zhu Baibi;Fu Xiaoping;Guo Jianchun;Jiang Xingyu
质膜（PM） Na+/H+反转运蛋白SOS1（盐过敏感1）是调控植物耐盐性的关键因子。SpSOS1基因编码PM Na+/H+反转运蛋白，从盐生植物麻瓜（Sesuvium portulacastrum）中克隆并转化到拟南芥（Arabidopsis） sos1突变植株中。结果表明，SpSOS1的表达与sos1突变体的盐敏感性互补。在盐胁迫下，spsos1转基因拟南芥sos1突变体种子的发芽率高于sos1突变体。在NaCl处理下，表达SpSOS1的sos1突变体植株生长良好，Na+/K+比值低于sos1突变体和野生型（WT）植株。此外，与盐胁迫下的sos1突变体和WT植株相比，spsos1过表达的拟南芥积累的丙二醛（MDA）更少，电解质泄漏水平也更低。此外，转基因植株中SpSOS1的表达也增加了一些盐胁迫相关基因的转录水平，如AtHKT1；1（高亲和K+转运蛋白1；1）、AtSOS2（盐过度敏感蛋白2）、atscasp8 （sos3样钙结合蛋白8）和AtNHX1 （Na+/H+交换蛋白1）。这些结果表明，SpSOS1通过调节盐胁迫下的离子稳态和保护质膜免受氧化损伤来提高植物的耐盐性。
The plasma membrane (PM) Na+/H+ antiporter SOS1 (salt overly sensitive 1) has emerged as a key factor in regulating plant salt tolerance. The SpSOS1 gene, which encodes a PM Na+/H+ antiporter, was cloned from the halophyte Sesuvium portulacastrum and transformed into Arabidopsis sos1 mutant plants. As shown from the results, the SpSOS1 expression complemented the salt sensitivity of the sos1 mutant plants. Upon salinity stress, SpSOS1-transgenic Arabidopsis sos1 mutant seeds displayed higher germination ratio compared to the sos1 mutant. The sos1 mutant plants expressing SpSOS1 grew better and had a lower Na+/K+ ratio than that of the sos1 mutant and wild-type (WT) plants when they were treated with NaCl. In addition, SpSOS1-overexpressed Arabidopsis accumulated less malondialdehyde (MDA) and had a lower level of electrolyte leakage than that in the sos1 mutant and WT plants under salt stress. Furthermore, the SpSOS1 expression in transgenic sos1 mutant plants also increased the transcript levels of some salt stress-related genes, such as AtHKT1;1 (high-affinity K+ transporter 1;1), AtSOS2 (salt overly sensitive 2), AtSCABP8 (SOS3-like calcium binding protein 8), and AtNHX1 (Na+/H+ exchanger 1). These results suggested that SpSOS1 improved the plant salt tolerance by regulating ion homeostasis and protecting the plasma membrane against oxidative damage under salt stress.
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影响因子：
2.9
作者：
Ma, Dong-Mei;Xu, Wei-Rong;Li, Hui-Wen;Jin, Feng-Xia;Guo, Ling-Na;Wang, Jing;Dai, Hong-Jun;Xu, Xing
通讯作者：
Xu, Xing
DOI：
10.1007/3-211-26392-6_75
发表时间：
1988
期刊：
--
影响因子：
--
作者：
Bill Walker Production
通讯作者：
Bill Walker Production
DOI：
10.1073/pnas.97.7.3730
发表时间：
2000-03
影响因子：
11.1
作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
通讯作者：
Jiping Liu;M. Ishitani;U. Halfter;Cheol-Soo Kim;Jian‐Kang Zhu
DOI：
10.1073/pnas.122224699
发表时间：
2002-06-11
影响因子：
11.1
作者：
Qiu, QS;Guo, Y;Zhu, JK
通讯作者：
Zhu, JK
DOI：
10.1073/pnas.0604711103
发表时间：
2006-12-05
影响因子：
11.1
作者：
Katiyar-Agarwal, Surekha;Zhu, Jianhua;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang