Streptococcal Opacity Factor: A Family of Bifunctional Proteins with Lipoproteinase and Fibronectin-Binding Activities
Streptococcal Opacity Factor: A Family of Bifunctional Proteins with Lipoproteinase and Fibronectin-Binding Activities
复制标题
链球菌混浊因子：具有脂蛋白酶和纤连蛋白结合活性的双功能蛋白家族
作者：
V. Katerov;Per;A. Totolian;C. Schalén
抽象。化脓性链球菌血清混浊因子（SOF）是一种类型特异性的脂蛋白酶，其生物学意义尚不清楚。我们已经测序了SOF基因，并从M63型菌株中鉴定了相应的SOF蛋白。发现sof63与sof22相关，并且与SOF22类似[25]，SOF63结合纤连蛋白。此外，我们证明了停乳链球菌纤连蛋白结合蛋白FnBA中的不透明因子活性，该蛋白在结构上与S.化脓序列分析表明，这三个SOF蛋白的保守区和可变区的独特的周期性模式。SOF63的酶活性部分定位于对应于整个保守和可变序列集的片段，而对于纤连蛋白结合，蛋白质的C末端部分中的单个重复是足够的。结果表明，链球菌SOF蛋白形成了一个新的家庭的双功能蛋白与脂蛋白酶和纤连蛋白结合活性。
Abstract. The serum opacity factor (SOF) of Streptococcus pyogenes is a type-specific lipoproteinase of unknown biological significance. We have sequenced the sof gene and characterized the corresponding SOF protein from a strain of type M63. It was found that sof63 is related to sof22 and that, similar to SOF22 [25], SOF63 binds fibronectin. Moreover, we demonstrate opacity factor activity in a Streptococcus dysgalactiae fibronectin-binding protein FnBA that is structurally related to the SOF proteins of S. pyogenes. Sequence analysis of these three SOF proteins showed a unique periodical pattern of conserved and variable regions. The enzymatically active part of SOF63 was localized to the fragment corresponding to the entire set of conserved and variable sequences, while for fibronectin-binding a single repeat in the C terminal part of the protein was sufficient. The results show that streptococcal SOF proteins form a novel family of bifunctional proteins with lipoproteinase and fibronectin-binding activities.
M蛋白和F蛋白是皮肤组织中化脓性链球菌细胞特异性趋向性的重要决定因素。
DOI：
10.1172/jci117463
发表时间：
1994
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Okada,N;Pentland,AP;Falk,P;Caparon,MG
通讯作者：
Caparon,MG
virR 在化脓性链球菌 M49 中的插入失活表明 VirR 作为 ScpA、FcRA、OF 和 M 蛋白的正调节因子发挥作用。
影响因子：
2.1
作者：
McLandsborough,LA;Cleary,PP
通讯作者：
Cleary,PP
DOI：
10.1016/s0021-9258(18)46035-8
发表时间：
1992-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Per-Eric Lindgren;Pietro Speziale;M. McGavin;H. Monstein;M. Höök;Livia Visai;Timo Kostiainen;S. Bozzini;Martin Lindberg
通讯作者：
Per-Eric Lindgren;Pietro Speziale;M. McGavin;H. Monstein;M. Höök;Livia Visai;Timo Kostiainen;S. Bozzini;Martin Lindberg