M protein and protein F act as important determinants of cell-specific tropism of Streptococcus pyogenes in skin tissue.
M protein and protein F act as important determinants of cell-specific tropism of Streptococcus pyogenes in skin tissue.
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M蛋白和F蛋白是皮肤组织中化脓性链球菌细胞特异性趋向性的重要决定因素。
DOI：
10.1172/jci117463
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Caparon,MG
中科院分区：
文献类型：
--
作者：
Okada,N;Pentland,AP;Falk,P;Caparon,MG
致病性革兰氏阳性细菌化脓性链球菌（A 组链球菌）会引起多种皮肤组织疾病，每种疾病都是在细菌与表皮细胞相互作用后引发的。在这项研究中，我们表明化脓性链球菌的不同表面蛋白在确定皮肤中细菌的细胞特异性趋向性方面发挥着重要作用。使用编码表面蛋白的基因中具有确定突变的链球菌菌株，与人类皮肤的原代培养物相结合，并使用人类皮肤的组织学切片进行原位粘附测定，我们发现化脓性链球菌的M蛋白介导细菌与角质形成细胞的结合，而第二种链球菌表面蛋白，蛋白F，指导生物体与朗格汉斯细胞的粘附。结合的表征表明，纯化的链球菌蛋白和用蛋白酶胰蛋白酶预处理两种宿主细胞可抑制粘附。体外角质形成细胞分化状态仅轻微影响粘附性，但会根据已知调节 M 蛋白和蛋白 F 表达的环境条件而受到相当大的调节，表明这些细菌细胞表面结构/粘附素与角质形成细胞和朗格汉斯细胞之间的相互作用可能在链球菌皮肤病中发挥重要作用。
The pathogenic gram-positive bacterium Streptococcus pyogenes (group A streptococcus) causes numerous diseases of cutaneous tissue, each of which is initiated after the interaction of the bacterium with the cells of the epidermis. In this study, we show that different surface proteins of S. pyogenes play important roles in determining the cell-specific tropism of the bacterium in skin. Using streptococcal strains with defined mutations in the genes which encode surface proteins in combination with primary cultures of human skin and an in situ adherence assay which uses histological sections of human skin, we show that the M protein of S. pyogenes mediates the binding of the bacterium to keratinocytes, while a second streptococcal surface protein, protein F, directs the adherence of the organism to Langerhans' cells. Characterization of binding revealed that adherence was inhibited by purified streptococcal proteins and pretreatment of both host cells with the protease trypsin. Adherence was only slightly affected by the state of keratinocyte differentiation in vitro, but was considerably modulated in response to environmental conditions known to regulate expression of M protein and protein F, suggesting that the interaction between these bacterial cell-surface structures/adhesins and keratinocytes and Langerhans' cells may play an important role in streptococcal skin disease.Images
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影响因子：
11.1
作者：
V. DiRita;J. Mekalanos
通讯作者：
V. DiRita;J. Mekalanos
影响因子：
6.4
作者：
BEACHEY, EH
通讯作者：
BEACHEY, EH
影响因子：
3.1
作者：
DEMAN, P;VANKOOTEN, C;EDEN, CS
通讯作者：
EDEN, CS
纤连蛋白与链球菌的相互作用：纤连蛋白作为化脓性链球菌受体的作用。
DOI：
10.1093/clinids/9.supplement_4.s351
发表时间：
1987
期刊：
Reviews of infectious diseases
影响因子：
--
作者：
Simpson,WA;Courtney,HS;Ofek,I
通讯作者：
Ofek,I
使用高碘酸盐氧化检测碳水化合物表位特异性单克隆抗体。
影响因子：
2.2
作者：
Woodward,MP;YoungJr,WW;Bloodgood,RA
通讯作者：
Bloodgood,RA