Bacteriophage therapy on the conchocelis of Pyropia haitanensis (Rhodophyta) infected by Vibrio mediterranei 117-T6
Bacteriophage therapy on the conchocelis of Pyropia haitanensis (Rhodophyta) infected by Vibrio mediterranei 117-T6
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噬菌体治疗地中海弧菌 117-T6 感染的坛紫菜 (红藻) 的壳状体
DOI：
10.1016/j.aquaculture.2020.735853
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发表时间：
2021-01
期刊：
影响因子：
4.5
通讯作者：
Chen Haimin
中科院分区：
文献类型：
--
作者：
Zhu Junkai;Xu Mengya;Liu Qiqin;Li Dengfeng;Yang Rui;Chen Haimin
黄斑病(Ysd)是一种常见于商品肉丝状体育苗阶段的破坏性病害，经鉴定，cultivatedPyropiaspecies.Vibriomediterranei117-T6是引起黄斑病的病原菌。为了建立YSD的生物防治方案，我们研究了噬菌体B_VMEM-永XC31(XC31噬菌体)对感染V的海棠果蝇的病原菌密度、自由生活丝状体(FLC)和壳传丝状体(SBC)的生理生化参数的影响。Medterranei117-T6。病毒密度最高时，FLC的存活率最高(83%)。MEDITERRANIE117-T6为108CFU/mL，噬菌体XC31的感染复数(MOI)为1。在此条件下，噬菌体XC31可杀灭病原菌，显著降低感染系统中的弧菌密度(P<；P<；P<0.05)。与XC31噬菌体共培养感染FLC的超氧化物歧化酶活性、藻蓝蛋白和别藻蓝蛋白含量显著高于未与XC31共培养的FLC(P<；P<0.05)。这些结果表明，XC31噬菌体能有效地控制由V引起的YSD。中草药117-T6通过杀灭病原菌和提高受感染的焦核盘藻的存活率来抑制焦核盘藻的生长。同时，噬菌体保护组提高了藻类的光合作用和抗氧化能力。因此，噬菌体疗法可以作为海藻养殖中细菌致病菌引起的疾病的一种替代有效的治疗方法。
Yellow spot disease (YSD) is a destructive disease that usually occurs at the conchocelis nursery stage of commercially cultivatedPyropiaspecies.Vibriomediterranei117-T6 has been identified as the pathogenic bacteria causing YSD. To develop a biological control program for YSD, we investigated the effects of bacteriophagevB_VmeM-YongXC31 (phage XC31) on the density of the pathogenic bacteria, the physiological and biochemical parameters of the free-living conchocelis (FLC) and the shell-borne conchocelis (SBC) ofPyropia haitanensisinfected byV. mediterranei117-T6. The highest survival rate (83%) of infected FLC was obtained when the density ofV. mediterranei117-T6 was 108CFU/mL and the multiplicity of infection (MOI) of phage XC31 was 1. Under these conditions, phage XC31 could kill the pathogens and significantly decrease theVibriodensity in the infection system (P< 0.05). The activity of superoxide dismutase, and the contents of phycocyanin and allophycocyanin in the infected FLC co-cultured with phage XC31 were significantly higher than those that were not co-cultured with phage XC31 (P< 0.05). These results indicated that phage XC31 could effectively control YSD caused byV. mediterranei117-T6 inPyropiaspecies by killing the pathogen and increasing the survival rate of infectedPyropiaconchocelis. Meanwhile, the algal photosynthesis and antioxidant capacity were improved in the bacteriophage protected groups. Hence, bacteriophage therapy can be used as an alternative and effective treatment for the diseases caused by bacterial pathogens in seaweed cultivation.
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