Plate heights below 50 nm for protein electrochromatography using silica colloidal crystals.
Plate heights below 50 nm for protein electrochromatography using silica colloidal crystals.
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DOI：
10.1021/ac102438w
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发表时间：
2010-12-15
影响因子：
7.4
通讯作者：
Wirth, Mary J.
中科院分区：
文献类型：
--
作者：
Wei, Bingchuan;Malkin, Douglas S.;Wirth, Mary J.
二氧化硅胶体晶体由75 μm i.d.评价熔融石英毛细管的蛋白质毛细管电色谱效率。三种蛋白质，核糖核酸酶A，细胞色素C和溶菌酶，每个共价标记的荧光团，以及通过等度电迁移，使用40：60的乙腈：水与0.1%的甲酸在1厘米的距离上分离。一个货车Deemter图显示，板高度的溶菌酶，这是最纯的三种蛋白质，是扩散限制的电场范围从400至1400 V/cm。在几乎所有的迁移速度下，溶菌酶的板高都小于50 nm，在最高速度下接近10 nm。涡流扩散可忽略不计。溶菌酶在1.5分钟内迁移超过106个平板的12 mm分离长度。这些结果表明，二氧化硅胶体晶体非常适合于大的，高电荷的分析物，如蛋白质的电驱动分离。观察到的106个板的分离长度仅超过1 cm，这意味着它们对于微芯片和μTAS设备中的小型化分离具有潜在价值。
Silica colloidal crystals formed from 330 nm nonporous silica spheres inside of 75-μm i.d. fused silica capillaries were evaluated for the efficiency of capillary electrochromatography of proteins. Three proteins, ribonuclease A, cytochrome C and lysozyme, each covalently labeled with fluorophor, were well separated over a distance of 1 cm by isocratic electromigration, using 40:60 acetonitrile:water with 0.1% formic acid. A van Deemter plot showed that the plate height for lysozyme, which was the purest of the three proteins, was diffusion-limited for electric fields ranging from 400 to 1400 V/cm. The plate height for lysozyme was below 50 nm at almost all of the migration velocities, and it approached 10 nm at the highest velocity. Eddy diffusion was negligible. Lysozyme migrated over a 12 mm separation length with more than 106 plates in 1.5 minutes. These results indicate that silica colloidal crystals are well suited for electrically driven separations of large, highly charged analytes such as proteins. The 106 plates observed for a separation length of barely more than a cm means they are potentially valuable for miniaturized separations in microchip and μTAS devices.
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影响因子：
7.4
作者：
MOORE, AW;JORGENSON, JW
通讯作者：
JORGENSON, JW
影响因子：
7.4
作者：
MacNair, JE;Lewis, KC;Jorgenson, JW
通讯作者：
Jorgenson, JW
涂有 C18 修饰金纳米颗粒的二氧化硅球，用于毛细管 LC 和加压 CEC 分离
影响因子：
2.9
作者：
Qu, Qishu;Peng, Shengwei;Yan, Chao
通讯作者：
Yan, Chao
DOI：
10.2174/138620709787315508
发表时间：
2009-02-01
影响因子：
1.8
作者：
Wright, James C.;Hubbard, Simon J.
通讯作者：
Hubbard, Simon J.
DOI：
10.1081/jlc-200053046
发表时间：
2005-01-01
影响因子：
1.3
作者：
Swartz, ME
通讯作者：
Swartz, ME