Insect pollination: an ecological process involved in the assembly of the seed microbiota
Insect pollination: an ecological process involved in the assembly of the seed microbiota
复制标题
昆虫授粉：涉及种子微生物群组装的生态过程
作者：
A. Prado;B. Marolleau;B. Vaissière;M. Barret;G. Torres
种子微生物群的组装涉及一些早期的微生物种子定殖者，它们通过维管系统从母体植物传播，而其他微生物则通过柱头进入。因此，种子微生物群不仅包括从植物维管组织招募的微生物，还包括从花中招募的微生物。众所周知，花是植物和昆虫之间微生物传播的中心。这种花与昆虫的交换为昆虫传播的细菌在胚珠上定居提供了可能性，随后，种子进入下一代植物。在这项研究中，我们通过高通量测序评估了昆虫授粉对种子微生物群的贡献。油菜（OSR）花暴露于蜜蜂（意大利蜜蜂），红梅森蜜蜂（壁蜂），人工授粉或自主自花授粉（ASP）的访问和授粉。序列分析表明，蜜蜂访问降低了种子中细菌的丰富度和多样性，但增加了种子微生物结构的变异性，并引入了蜜蜂相关的类群。相比之下，梅森蜜蜂授粉对种子微生物群的影响较小。我们的研究提供了第一个证据，表明昆虫授粉是一个生态过程，涉及细菌从花朵到种子的传播。
The assembly of the seed microbiota involves some early microbial seed colonizers that are transmitted from the maternal plant through the vascular system, while other microbes enter through the stigma. Thus, the seed microbiota consists of microbes not only recruited from the plant vascular tissues, but also from the flower. Flowers are known to be a hub for microbial transmission between plants and insects. This floral-insect exchange opens the possibility for insect-transmitted bacteria to colonize the ovule and, subsequently, the seed to pass then into the next plant generation. In this study, we evaluated the contribution of insect pollination to the seed microbiota through high-throughput sequencing. Oilseed rape (OSR) flowers were exposed to visits and pollination by honey bees (Apis mellifera), red mason bees (Osmia bicornis), hand pollinated or left for autonomous self-pollination (ASP). Sequence analyses revealed that honey bee visitation reduced bacterial richness and diversity in seeds, but increased the variability of seed microbial structure, and introduced bee-associated taxa. In contrast, mason bee pollination had minor effects on the seed microbiota. Our study provides the first evidence that insect pollination is an ecological process involved in the transmission of bacteria from flowers to seeds.
影响因子：
1.6
作者：
Willmer, P. G.;Cunnold, H.;Ballantyne, G.
通讯作者：
Ballantyne, G.
DOI：
10.1073/pnas.1505765112
发表时间：
2015-09-08
影响因子：
11.1
作者：
Santhanam, Rakesh;Van Thi Luu;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.
影响因子：
5.1
作者：
Manirajan, Binoy Ambika;Ratering, Stefan;Schnell, Sylvia
通讯作者：
Schnell, Sylvia