Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples.
Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples.
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DOI：
10.1371/journal.pone.0017549
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发表时间：
2011-03-03
期刊：
影响因子：
3.7
通讯作者：
Muniesa M
中科院分区：
文献类型：
--
作者：
Colomer-Lluch M;Jofre J;Muniesa M
抗生素耐药性是一个日益严重的全球性问题，这是由于抗生素使用的压力、人口的更大流动性和工业化造成的。许多抗生素抗性基因被认为起源于环境中的微生物，并通过移动的遗传元件转移到其他细菌。其中，β-内酰胺类抗生素显示出临床疗效和低毒性，因此它们被广泛用作抗微生物剂。对β-内酰胺类抗生素的耐药性是由β-内酰胺酶基因和青霉素结合蛋白（由染色体或质粒编码）引起的，尽管关于其他移动的遗传因子（如β-内酰胺酶）的作用信息很少。本研究的重点是从环境水样中分离的噬菌体DNA中赋予β-内酰胺抗生素抗性的三个基因，即两个β-内酰胺酶基因（blaTEM和blaCTX-M9）和一个编码青霉素结合蛋白（mecA）的基因。从30个城市污水和河水样品中收集的噬菌体分离的DNA中，使用定量PCR扩增的三个基因进行定量。所有三种基因均在来自所有测试样品的大肠杆菌DNA中检测到，在某些情况下达到blaTEM的104个基因拷贝（GC）或blaCTX-M和mecA的102个GC。这些值与样品中粪便污染的量一致，除了mecA，其在河水样品中的拷贝数高于城市污水。通过电穿孔将来自噬菌体DNA的bla基因转移到敏感的宿主菌中，所述宿主菌变得对氨苄青霉素具有抗性。转染后的抗性克隆DNA中检测到blaTEM和blaCTX。这项研究表明，水稻是环境中抗性基因的储存库。
Antibiotic resistance is an increasing global problem resulting from the pressure of antibiotic usage, greater mobility of the population, and industrialization. Many antibiotic resistance genes are believed to have originated in microorganisms in the environment, and to have been transferred to other bacteria through mobile genetic elements. Among others, β-lactam antibiotics show clinical efficacy and low toxicity, and they are thus widely used as antimicrobials. Resistance to β-lactam antibiotics is conferred by β-lactamase genes and penicillin-binding proteins, which are chromosomal- or plasmid-encoded, although there is little information available on the contribution of other mobile genetic elements, such as phages. This study is focused on three genes that confer resistance to β-lactam antibiotics, namely two β-lactamase genes (blaTEM and blaCTX-M9) and one encoding a penicillin-binding protein (mecA) in bacteriophage DNA isolated from environmental water samples. The three genes were quantified in the DNA isolated from bacteriophages collected from 30 urban sewage and river water samples, using quantitative PCR amplification. All three genes were detected in the DNA of phages from all the samples tested, in some cases reaching 104 gene copies (GC) of blaTEM or 102 GC of blaCTX-M and mecA. These values are consistent with the amount of fecal pollution in the sample, except for mecA, which showed a higher number of copies in river water samples than in urban sewage. The bla genes from phage DNA were transferred by electroporation to sensitive host bacteria, which became resistant to ampicillin. blaTEM and blaCTX were detected in the DNA of the resistant clones after transfection. This study indicates that phages are reservoirs of resistance genes in the environment.
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影响因子：
3.7
作者：
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通讯作者：
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影响因子：
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通讯作者：
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作者：
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