Recombinant adenovirus-mediated intestinal trefoil factor gene therapy for burn-induced intestinal mucosal injury.
Recombinant adenovirus-mediated intestinal trefoil factor gene therapy for burn-induced intestinal mucosal injury.
复制标题
重组腺病毒介导的肠三叶因子基因治疗烧伤引起的肠粘膜损伤。
DOI：
10.1371/journal.pone.0062429
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peng Y
中科院分区：
文献类型：
--
作者：
Sun Y;Zhu Y;Wang L;Mao X;Peng X;Peng Y
肠三叶因子（Intestinal trefoil factor， ITF）是一种具有潜在医学价值的小多肽，其主要药理作用是减轻各种损伤因素引起的胃肠道粘膜损伤，促进受损粘膜的修复。但其产量低，限制了其应用。本研究的目的是构建含hITF基因的重组腺病毒载体，通过体外和体内分析观察其对烧伤性肠黏膜损伤的治疗效果。首先，将含有信号肽和成熟肽的全长hITF基因与pAdTrack-CMV载体连接构建重组穿梭质粒，然后与BJ5183株主质粒进行线性化和同源重组，构建重组Ad-hITF腺病毒载体。其次，采用逆转录聚合酶链反应和western blotting检测Ad-hITF感染结肠癌HT-29细胞后hITF的表达水平。重组腺病毒在体外损伤模型中显著促进细胞迁移。最后，我们通过建立小鼠重度烧伤模型，通过胃内注射重组腺病毒，证实重组腺病毒能够显著促进肠黏膜损伤的愈合。综上所述，我们构建了含有hITF基因的重组腺病毒载体，并证实了其促进肠黏膜修复的作用。本研究为烧伤性肠黏膜损伤的治疗提供了一种新的途径。
Intestinal trefoil factor (ITF) is a small polypeptide with potential medical values whose main pharmacological effects are to alleviate gastrointestinal mucosal injury caused by various injury factors and promote the repair of damaged mucosa. However, its low yield limits its application. The purpose of our study was to construct a recombinant adenoviral vector containing the hITF gene and observe the therapeutic effect of burn-induced intestinal mucosal injury using in vitro and in vivo analysis. First, a recombinant shuttle plasmid was constructed by ligating a pAdTrack-CMV vector with a full-length hITF gene containing a signal peptide and the mature peptide, followed by the recombinant Ad-hITF adenovirus vector after linearization and homologous recombination with the backbone plasmid in the competent BJ5183 strain. Second, the hITF expression level was detected using reverse transcription polymerase chain reaction and western blotting after Ad-hITF infection of colon cancer HT-29 cells. The recombinant adenovirus significantly promoted cell migration in an in vitro wounding model. Finally, we confirmed that the recombinant adenovirus could significantly expedite the healing of intestinal mucosal injury after establishing a mouse model in which severe burns were stimulated and the recombinant adenovirus was delivered by intragastric injection. In summary, we constructed a recombinant adenoviral vector containing the hITF gene and confirmed its role in promoting repair of the intestinal mucosa. Our study provides a novel way to treat burn-induced intestinal mucosal injury.
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影响因子：
3.5
作者：
THIM, L
通讯作者：
THIM, L
影响因子：
4.2
作者：
Mizuguchi, H;Kay, MA
通讯作者：
Kay, MA
DOI：
10.4161/bbug.1.4.11725
发表时间：
2010-07-01
期刊：
Bioengineered bugs
影响因子：
--
作者：
Tangney, Mark;van Pijkeren, Jan Peter;Gahan, Cormac G M
通讯作者：
Gahan, Cormac G M
DOI：
10.1006/bbrc.1997.7144
发表时间：
1997-08-28
影响因子：
3.1
作者：
Tan, XD;Hsueh, W;GonzalezCrussi, F
通讯作者：
GonzalezCrussi, F
影响因子：
24.5
作者：
Millar, AD;Rampton, DS;Blake, DR
通讯作者：
Blake, DR