Head muscle fibro-adipogenic progenitors account for the tilted regeneration towards fibrosis.
Head muscle fibro-adipogenic progenitors account for the tilted regeneration towards fibrosis.
复制标题
头部肌肉纤维脂肪祖细胞导致再生向纤维化倾斜。
DOI：
10.1016/j.bbrc.2021.12.009
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发表时间：
2021
影响因子：
3.1
通讯作者：
Bing Shi
中科院分区：
文献类型：
--
作者：
Xu Cheng;Yixuan Huang;Yingmeng Liu;Jinfeng Dou;Ning Zhao;Jingtao Li;Bing Shi
鳃节头肌与体节肢肌在个体发育和胚胎发育上不同，它们表现出不同的再生能力。头部肌肉独特的卫星细胞特性可以解释受损的肌纤维形成，但纤维化的潜在机制仍然是难以捉摸的。在这项工作中，我们首先建立了冷冻诱导的骨骼肌再生模型，并比较了胫骨前肌（TA）和咬肌（MAS）的再生特性。通过组织学、免疫组化和细胞学分析，研究肌卫星细胞（MuSCs）和成纤维脂肪祖细胞（FAPs）在TA和MAS肌肉再生中的作用。我们的研究结果表明，FAPs在MAS肌肉再生过程中浸润纤维化区域。与TA肌FAP数量在早期再生阶段的快速上升和下降相反，MAS FAP数量在损伤后14天增加到一个平台而没有下降。这是第一次发现FAPs在头部肌肉再生中的关键作用。FAP在再生的前两周内没有及时清除的持续存在可能是导致头部肌肉纤维化的原因。
Branchiomeric head muscle is ontogenetically and phylogenetically distinct from somitic limb muscle, and they exhibit different regenerative capacity. Unique satellite cell property of head muscle could explain the impaired myofiber formation, but the underlying mechanism for fibrosis is still elusive. In this work, we first established a freezing-induced skeletal muscle regeneration model and made comparisons between the regeneration characteristics in tibialis anterior (TA) muscle and masseter (MAS) muscle. The process of myogenesis and fibrogenesis were investigated by histological, immunohistochemical and cellular analysis, to characterize the role of muscle satellite cell (MuSCs) and fibro-adipogenic progenitors (FAPs) in TA and MAS muscle regeneration. Our results revealed that FAPs infiltrated the fibrotic area during MAS muscle regeneration. In contrast to the rapid rise and fall of FAPs number at the early regeneration stages in TA muscle, the number of MAS FAPs increased to a plateau without descending till 14 days after injury. It is the first time that the pivotal role of FAPs in head muscle regeneration was characterized. The persistence of FAPs without timely clearance in the first two weeks of regeneration could be accountable for the head muscle fibrosis.
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