Feedback controls and G2 checkpoints: Fission yeast as a model system
Feedback controls and G2 checkpoints: Fission yeast as a model system
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反馈控制和 G2 检查点：裂殖酵母作为模型系统
作者：
K. Sheldrick;A. Carr
细胞周期内的依赖关系允许细胞响应于干扰其正常进展的特定方面的试剂或条件而可逆地阻止周期。此外，存在重叠途径，其也响应于DNA损伤而阻止细胞周期。这些控制机制统称为检查点。细胞周期内的依赖关系，如有丝分裂对DNA合成完成的依赖性，以及DNA损伤检查点可以在遗传上分开，这一事实促进了检查点的分析。在裂殖酵母中，有丝分裂对DNA合成的依赖性是通过普遍存在的有丝分裂调节因子p34 cdc 2的酪氨酸-15磷酸化介导的。相比之下，DNA损伤引起的有丝分裂停滞通过一种独立的机制起作用，似乎与酪氨酸-15磷酸化无关。尽管与有丝分裂机制有这些不同的相互作用，但大多数在DNA损伤后缺乏有丝分裂停滞的裂殖酵母突变体也不能对DNA合成的抑制作出反应。在这篇文章中，我们调查目前的知识反馈控制和检查点内裂变酵母和相关的信息来自其他系统。
Dependency relationships within the cell cycle allow cells to arrest the cycle reversibly in response to agents or conditions that interfere with specific aspects of its normal progression. In addition, overlapping pathways exist which also arrest the cell cycle in response to DNA damage. Collectively, these control mechanisms have become known as checkpoints. Analysis of checkpoints is facilitated by the fact that dependency relationships within the cell cycle, such as the dependency of mitosis on the completion of DNA synthesis, and the DNA damage checkpoint can be separated genetically. In fission yeast, Schizosaccharomyces pombe, the dependency of mitosis on prior completion of DNA synthesis is mediated through tyrosine‐15 phosphorylation of the ubiquitous mitotic regulator p34cdc2. In contrast, the arrest of mitosis caused by DNA damage acts through a separate mechanism that appears to be independent of tyrosine‐15 phosphorylation. Despite these distinct interactions with the mitotic machinery, the majority of fission yeast mutants that are deficient in mitotic arrest after DNA damage are also unable to respond to inhibition of DNA synthesis. In this essay we survey the current knowledge concerning feedback controls and checkpoints within fission yeast and relate this to information derived from other systems.
影响因子：
11.4
作者：
ROWLEY, R;SUBRAMANI, S;YOUNG, PG
通讯作者：
YOUNG, PG
DOI：
10.1073/pnas.87.15.5672
发表时间：
1990-08-01
影响因子：
11.1
作者：
LEE, SH;HURWITZ, J
通讯作者：
HURWITZ, J
影响因子：
56.9
作者：
HARTWELL, LH;WEINERT, TA
通讯作者：
WEINERT, TA