Identification of MAM1s in Regulation of 3C Glucosinolates Accumulation in Allopolyploid Brassica juncea
Identification of MAM1s in Regulation of 3C Glucosinolates Accumulation in Allopolyploid Brassica juncea
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异源多倍体芥菜中 MAM1 调节 3C 芥子油苷积累的鉴定
DOI：
10.1016/j.hpj.2020.10.001
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发表时间：
2020-10
影响因子：
5.7
通讯作者：
Yang Jinghua
中科院分区：
文献类型：
--
作者：
Li Zhangping;Zhang Changtong;Cai Qingze;Zhou Yulan;Hu Zhongyuan;Zhang Mingfang;Yang Jinghua
异源多倍体芥菜特别富含芥子苷，一种3C脂肪族硫代葡萄糖苷（GSL），采摘后产生特征味道。然而，该物种中3C脂肪族GSL生物合成的分子机制仍然未知。在本研究中，我们在全基因组范围内鉴定了GSL代谢基因，表明B亚基因组之间GSL代谢基因的进化速率不同。Juncea。从B中鉴定出8个甲基硫代烷基苹果酸合酶（MAMs）同源物。juncea中有6个MAM 1定位于叶绿体中，另外2个MAM 1不表达。此外，BjMAM 1 -4、BjMAM 1 -5和BjMAM 1 -6在叶片中的表达水平高于其他组织。沉默表达分析显示BjMAM 1 -4和BjMAM 1 -6在3C和4C脂肪族GSL积累中起作用。第二个循环反应的底物选择特异性远低于第一个循环，表明这些基因可能优先催化3C脂肪族GSL的生物合成。我们的研究提供了深入的分子机制的基础上积累的3C脂肪GSL，从而促进脂肪GSL含量的操纵B。Juncea。
Allopolyploid Brassica juncea is particularly enriched in sinigrin, a kind of 3C aliphatic glucosinolates (GSLs), giving rise to characteristic taste after picking. However, the molecular mechanism underlying 3C aliphatic GSLs biosynthesis in this species remains unknown. In this study, we genome-widely identified GSLs metabolic genes, indicating different evolutionary rate of GSLs metabolic genes between subgenomes of B. juncea. Eight methythioalkylmalate synthase (MAMs) homologs were identified from B. juncea, in which six MAM1s were located in chloroplast and the other two were not detected with any expression. Furthermore, BjMAM1-4, BjMAM1-5, and BjMAM1-6 displayed higher expression levels in leaves than other tissues. Silenced expression analysis revealed that BjMAM1-4 and BjMAM1-6 function in 3C and 4C aliphatic GSLs accumulation. The specificity of the substrate selection for the second cycle reaction is much lower than that of the first cycle, suggesting these genes may preferentially catalyze 3C aliphatic GSLs biosynthesis. Our study provides insights into the molecular mechanism underlying the accumulation of 3C aliphatic GSLs, thereby facilitating the manipulation of aliphatic GSLs content in B. juncea.
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影响因子：
9.4
作者：
Gigolashvili, Tamara;Engqvist, Martin;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
3.5
作者：
Wang, Hui;Wu, Jian;Wang, Xiaowu
通讯作者：
Wang, Xiaowu
影响因子：
7.4
作者：
Celenza, JL;Quiel, JA;Bender, J
通讯作者：
Bender, J
影响因子：
7.2
作者：
Gigolashvili, Tamara;Berger, Bettina;Fluegge, Ulf-Ingo
通讯作者：
Fluegge, Ulf-Ingo
影响因子：
7.2
作者：
Pflieger, Stephanie;Blanchet, Sandrine;Jupin, Isabelle
通讯作者：
Jupin, Isabelle