In vitro differentiation of bone marrow derived porcine mesenchymal stem cells to endothelial cells.
In vitro differentiation of bone marrow derived porcine mesenchymal stem cells to endothelial cells.
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DOI：
10.1002/term.1483
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发表时间：
2013-11
影响因子：
3.3
通讯作者：
Agrawal, Devendra K.
中科院分区：
文献类型：
--
作者：
Pankajakshan, Divya;Kansal, Vikash;Agrawal, Devendra K.
骨髓间充质干细胞（Mesenchymal stem cells，MSCs）具有修复心血管疾病损伤组织的潜能。在这项研究中，我们研究了猪骨髓间充质干细胞在体外分化为内皮细胞（EC）的潜力。培养的骨髓源性细胞为CD 11b − CD 34 − CD 44 + CD 45 − CD 90+，并显示出MSC特征性的中胚层谱系分化。使用含有和不含高浓度VEGF的内皮生长培养基（EGM+VEGF; 50 ng/ml）诱导MSC分化为EC。不含生长因子的内皮基础培养基（EBM）作为对照。刺激10天后，通过vWF的存在、摄取乙酰化LDL的能力、体外血管生成测定、流式细胞术和EC标志物vWF、VE-钙粘蛋白、PECAM-1、CD 34、VEGF-R1和VEGF-R2的qPCR来评估EC分化。EGM+VEGF培养液中DiI-AcLDL阳性细胞数明显高于EBM培养液，vWF（90%）、VE-Cadherin（60%）和PECAM-1（48%）阳性细胞数明显增加。这些细胞在血管生成试验中显示大量的毛细血管出芽和闭合多边形形成。与对照相比，EGM+VEGF培养基刺激的细胞中内皮标志物的mRNA表达增加1.5-2倍。结果证明了猪MSC在体外诱导条件下分化为EC的能力。分化的细胞将为介入手术和组织工程后的再内皮化提供新的选择。
Mesenchymal stem cells (MSCs) hold potential for the regeneration of damaged tissues in cardiovascular diseases. In this study, we investigated the potential of porcine MSCs to differentiate into endothelial cells (ECs) in-vitro. The cultured bone marrow derived cells were CD11b−CD34−CD44+CD45−CD90+ and showed mesodermal lineage differentiation which are characteristic of MSCs. The MSCs were induced to differentiate into ECs using endothelial growth media (EGM) with and without high concentrations of VEGF (EGM+VEGF; 50ng/ml). The endothelial basal medium (EBM) without growth factors served as the control. The EC differentiation was assessed by the presence of vWF, ability to take up acetylated LDL, in vitro angiogenesis assay, flow cytometry and qPCR of EC markers vWF, VE-cadherin, PECAM-1, CD34, VEGF-R1 and VEGF-R2 after 10days of stimulation. The cells cultured in EGM+VEGF media demonstrated higher amount of DiI-AcLDL positive cells, and enhanced presence of vWF (90%), VE-Cadherin (60%) and PECAM-1 (48%) positive cells, than in EBM. These cells showed profuse sprouting of capillary tubes and closed polygon formation in the angiogenesis assay. There was 1.5–2 fold increase in the mRNA expression of endothelial markers in the cells stimulated with EGM+VEGF media when compared to control. The results demonstrate the ability of porcine MSCs to differentiate into ECs under in-vitro inducing conditions. The differentiated cells would provide new options for re-endothelialization following interventional procedures and tissue engineering.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
5.3
作者：
Xu J;Liu X;Jiang Y;Chu L;Hao H;Liua Z;Verfaillie C;Zweier J;Gupta K;Liu Z
通讯作者：
Liu Z
影响因子：
20.3
作者：
Lee, OK;Kuo, TK;Chen, TH
通讯作者：
Chen, TH
DOI：
10.1073/pnas.032074999
发表时间：
2002-04-02
影响因子：
11.1
作者：
Levenberg, S;Golub, JS;Langer, R
通讯作者：
Langer, R
影响因子：
5.2
作者：
Oswald, J;Boxberger, S;Werner, C
通讯作者：
Werner, C