Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
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DOI：
10.1016/j.vetmic.2011.10.017
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发表时间：
2012-05-04
影响因子：
3.3
通讯作者：
Brown DR
中科院分区：
文献类型：
--
作者：
Brosnahan AJ;Brown DR
IPEC-J2细胞是从新生仔猪空肠中段分离的猪肠柱状上皮细胞。该细胞系在0.4 μ m孔径过滤器上培养时形成具有高跨上皮电阻的极化单层。该细胞系的独特之处在于其来源于小肠组织（与常见的人结肠来源的细胞系HT-29、T84和Caco-2相比），并且未转化（与猪小肠细胞系IPI-2I相比）。猪肠上皮细胞比类似的啮齿动物细胞系（例如IEC-6或IEC-18）更接近于模拟人类生理学，这在人畜共患病感染的研究中很重要;此外，它们为研究猪源性感染提供了特异性。IPEC-J2细胞越来越多地用于微生物学研究，以检查各种动物和人类病原体（包括肠道沙门氏菌和致病性大肠杆菌）与肠上皮细胞的相互作用。IPEC-J2细胞系也已用于一些益生菌研究，其中细胞已用作潜在益生菌微生物的抗炎和抗炎特性的初始筛选工具。这些研究的有效性尚不清楚，因为迄今为止尚未发表评估益生菌体内功效的后续研究。本次审查的目的是提供一个全面的概述已经进行了与IPEC-J2细胞的微生物研究和参考指南的关键细胞和免疫标志物，已确定在这个细胞系，可能被证明是有用的，在未来的研究。
IPEC-J2 cells are porcine intestinal columnar epithelial cells that were isolated from neonatal piglet mid-jejunum. This cell line forms polarized monolayers with high transepithelial electrical resistance when cultured on 0.4 μm pore-size filters. The cell line is unique in that it is derived from small intestinal tissue (compared to the common human colon-derived lines HT-29, T84, and Caco-2) and is not transformed (compared to the porcine small intestinal line, IPI-2I). Porcine intestinal epithelial cells more closely mimic human physiology than analogous rodent cell lines (e.g. IEC-6 or IEC-18), which is important in studies of zoonotic infections; in addition, they provide specificity to study porcine-derived infections. IPEC-J2 cells are increasingly being used in microbiological studies to examine the interactions of various animal and human pathogens, including Salmonella enterica and pathogenic Escherichia coli, with intestinal epithelial cells. The IPEC-J2 cell line has also been employed in some probiotic studies, in which the cells have been used as an initial screening tool for adhesiveness and anti-inflammatory properties of the potential probiotic microorganisms. The validity of these studies is not clear as follow-up studies to assess the efficacy of the probiotics in vivo have not been published to date. The aims of this review are to provide a comprehensive overview of the microbiological studies that have been conducted with IPEC-J2 cells and a reference guide of key cellular and immune markers that have been identified in this cell line that may prove to be useful in future studies.
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影响因子：
3.5
作者：
Diesing, Anne-Kathrin;Nossol, Constanze;Kahlert, Stefan
通讯作者：
Kahlert, Stefan
DOI：
10.1152/ajpgi.1995.269.4.g591
发表时间：
1995-10-01
影响因子：
4.5
作者：
KANDIL, HM;ARGENZIO, RA;RHOADS, JM
通讯作者：
RHOADS, JM
影响因子：
3.3
作者：
IMBERECHTS, H;BERTSCHINGER, HU;LINTERMANS, P
通讯作者：
LINTERMANS, P
影响因子：
2.4
作者：
Boyen, F.;Pasmans, F.;Haesebrouck, F.
通讯作者：
Haesebrouck, F.
影响因子：
2.1
作者：
Brown, Eric A.;Kaushik, Radhey S.;Hardwidge, Philip R.
通讯作者：
Hardwidge, Philip R.