Gut Microbiota and Relevant Metabolites Analysis in Alcohol Dependent Mice.
Gut Microbiota and Relevant Metabolites Analysis in Alcohol Dependent Mice.
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酒精依赖小鼠的肠道微生物群和相关代谢物分析
DOI：
10.3389/fmicb.2018.01874
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发表时间：
2018
影响因子：
5.2
通讯作者：
Pan J
中科院分区：
文献类型：
--
作者：
Wang G;Liu Q;Guo L;Zeng H;Ding C;Zhang W;Xu D;Wang X;Qiu J;Dong Q;Fan Z;Zhang Q;Pan J
酗酒是一项重大的公共健康危机。相关证据支持肠道微生物区系(GM)在中枢神经系统(CNS)功能中起着重要作用，饮酒后肠道微生物区系的组成发生了变化。本研究旨在探讨酒精依赖时GM的变化。本研究通过分析16S rRNA基因序列，检测酒精灌胃小鼠的GM，并用LC-MS分析其粪便代谢物。酒精染毒组微生物多样性显著增加，梭状芽胞菌门和梭状芽孢杆菌的丰度增加，泽泻科、泽泻属和臭杆菌的丰度在三组间有显著差异。根据LC-MS结果，酒精模型大鼠的胆汁酸、次级胆汁酸、5-羟色胺和牛磺酸水平均有不同程度的变化。石蜡切片可见肝、结肠组织损伤。这些发现为酒精摄入影响GM的组成提供了直接证据，使人们能够更好地了解GM在微生物-肠道-脑(MGB)轴上的功能，并为酒精成瘾的治疗提供了新的思路。
Alcohol abuse is a major public health crisis. Relative evidences supported that the gut microbiota (GM) played an important role in central nervous system (CNS) function, and the composition of them had changed after alcohol drinking. We sought to explore the changes of GM in alcohol dependence. In our study, the GM of mice with alcohol administration was detected through analyzed 16S rRNA gene sequencing and the fecal metabolites were analyzed by LC-MS. The microbial diversity was significantly higher in the alcohol administration group, the abundance of phylum Firmicutes and its class Clostridiales were elevated, meanwhile the abundance of Lachnospiraceae, Alistipes, and Odoribacter showed significant differences among the three groups. Based on LC-MS results, bile acid, secondary bile acid, serotonin and taurine level had varying degrees of changes in alcohol model. From paraffin sections, tissue damage was observed in liver and colon. These findings provide direct evidence that alcohol intake affects the composition of GM, enable a better understanding of the function of GM in the microbiota-gut-brain (MGB) axis, and give a new thought for alcohol addiction treatment.
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DOI：
10.1016/j.pnpbp.2015.07.011
发表时间：
2016-02-04
影响因子：
5.6
作者：
Berrettini, Wade
通讯作者：
Berrettini, Wade
一类重要的微生物胆汁酸的生物合成途径。
影响因子：
14.8
作者：
Devlin AS;Fischbach MA
通讯作者：
Fischbach MA
影响因子：
29.4
作者：
Alemi F;Poole DP;Chiu J;Schoonjans K;Cattaruzza F;Grider JR;Bunnett NW;Corvera CU
通讯作者：
Corvera CU
影响因子：
64.5
作者：
Bellono NW;Bayrer JR;Leitch DB;Castro J;Zhang C;O'Donnell TA;Brierley SM;Ingraham HA;Julius D
通讯作者：
Julius D
影响因子：
2.9
作者：
Filiano, Anthony J.;Gadani, Sachin P.;Kipnis, Jonathan
通讯作者：
Kipnis, Jonathan