Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales.
Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales.
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DOI：
10.1016/j.actbio.2013.08.038
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发表时间：
2014-04
影响因子：
9.7
通讯作者：
Stegemann, J. P.
中科院分区：
文献类型：
--
作者：
Walters, B. D.;Stegemann, J. P.
I型胶原蛋白是一种广泛使用的天然生物材料，已发现其在多种生物和医学应用中的效用。其良好表征的结构和作为细胞外基质蛋白的作用使其成为用于控制细胞功能和模拟组织特性的高度相关的材料。I型胶原蛋白在许多组织中是丰富的，并且可以作为纯化的蛋白质分离。本文综述了水凝胶生物材料的重建I型胶原蛋白从溶解的形式，重点是在体外研究中，胶原蛋白的结构可以控制。胶原蛋白的层次结构从纳米级到宏观尺度进行了描述，强调结构如何与跨尺度的功能相关。介绍了将胶原蛋白重组成水凝胶材料的方法，包括宏观结构的成型、微尺度模块的创建和纳米级纤维的静电纺丝。胶原蛋白生物材料的改性，以实现所需的结构和功能也得到了解决，特别强调胶原蛋白结构的机械控制，胶原蛋白复合材料的创建，和胶原基质的交联。生物材料科学家在合理设计胶原基生物材料并将其应用于生物学研究和治疗方面取得了显着进展。这一广泛的审查说明了最近的例子，用于控制胶原蛋白结构的技术，从而指导其生物和机械功能。
Collagen type I is a widely used natural biomaterial that has found utility in a variety of biological and medical applications. Its well characterized structure and role as an extracellular matrix protein make it a highly relevant material for controlling cell function and mimicking tissue properties. Collagen type I is abundant in a number of tissues, and can be isolated as a purified protein. This review focuses on hydrogel biomaterials made by reconstituting collagen type I from a solubilized form, with an emphasis on in vitro studies in which collagen structure can be controlled. The hierarchical structure of collagen from the nanoscale to the macroscale is described, with an emphasis on how structure is related to function across scales. Methods of reconstituting collagen into hydrogel materials are presented, including molding of macroscopic constructs, creation of microscale modules, and electrospinning of nanoscale fibers. The modification of collagen biomaterials to achieve desired structures and functions is also addressed, with particular emphasis on mechanical control of collagen structure, creation of collagen composite materials, and crosslinking of collagenous matrices. Biomaterials scientists have made remarkable progress in rationally designing collagen-based biomaterials and in applying them to both the study of biology and for therapeutic benefit. This broad review illustrates recent examples of techniques used to control collagen structure, and to thereby direct its biological and mechanical functions.
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影响因子：
5
作者：
Achilli, Matteo;Mantovani, Diego
通讯作者：
Mantovani, Diego
影响因子：
8
作者：
Berisio, R;Vitagliano, L;Zagari, A
通讯作者：
Zagari, A
DOI：
10.1115/1.2796072
发表时间：
1997-05-01
影响因子：
1.7
作者：
Barocas, VH;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
4.1
作者：
Ananta, M.;Aulin, Cecilia E.;Mudera, Vivek
通讯作者：
Mudera, Vivek
影响因子：
14
作者：
Buttafoco, L;Kolkman, NG;Feijen, J
通讯作者：
Feijen, J