Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice.
Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice.
复制标题
DOI：
10.1016/j.bbi.2012.12.014
复制
发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Woods JA
中科院分区：
文献类型：
--
作者：
Martin SA;Pence BD;Greene RM;Johnson SJ;Dantzer R;Kelley KW;Woods JA
脂多糖对天然免疫系统的外周刺激会导致老年小鼠神经炎症加重和疾病行为延长。有规律的中等强度运动已被证明可以发挥抗炎作用，可能会保护老年小鼠免受不适当的神经炎症和疾病。本研究的目的是验证这样一种假设，即随意的车轮运动可以减轻22月龄C57BL/6J小鼠的内毒素诱导的疾病行为和促炎细胞因子的基因表达。将小鼠分成有车轮(VWR)、带锁车轮(LOCKED)和无车轮(STANDARD)3组，每组10周，然后按不同剂量(0.02、0.08、0.16、0.33 mg/kg)腹腔注射脂多糖。与锁住和鞋盒的小鼠相比，VWR小鼠平均每天跑3.5公里，体重和体脂减少明显，并增加了它们的强迫运动耐力。与上锁组和鞋盒组相比，VWR在任何剂量下对脂多糖诱导的厌食、肥胖、体重减轻或运动活动减少没有影响。脂多糖以剂量依赖的方式诱导小鼠的疾病行为(0.33&0.02 mg/kg)。注射0.33 mg/kg内毒素或生理盐水后24小时，内毒素诱导的大鼠全脑肿瘤坏死因子α、IL-1β和IL-10β表达上调，脾、肝组织IL-1和IL-6表达增加，但VWR不能减弱这些作用。我们的结论是，VWR不能减少内毒素诱导的老年动物夸大或延长的疾病行为，也不能减少注射(0.33 mg/kg或生理盐水)后24小时脑和外周促炎细胞因子的基因表达。疾病反应的必要性对生存至关重要，可能会超过运动训练对老年动物的微妙好处。
Peripheral stimulation of the innate immune system with LPS causes exaggerated neuroinflammation and prolonged sickness behavior in aged mice. Regular moderate intensity exercise has been shown to exert anti-inflammatory effects that may protect against inappropriate neuroinflammation and sickness in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running would attenuate LPS-induced sickness behavior and proinflammatory cytokine gene expression in ~22-month-old C57BL/6J mice. Mice were housed with a running wheel (VWR), locked-wheel (Locked), or no wheel (Standard) for 10 weeks, after which they were intraperitoneally injected with LPS across a range of doses (0.02, 0.08, 0.16, 0.33 mg/kg). VWR mice ran on average 3.5 km/day and lost significantly more body weight and body fat, and increased their forced exercise tolerance compared to Locked and Shoebox mice. VWR had no effect on LPS-induced anorexia, adipsia, weight-loss, or reductions in locomotor activity at any LPS dose when compared to Locked and Shoebox groups. LPS induced sickness behavior in a dose-dependent fashion (0.33>0.02 mg/kg). Twenty-four hours post-injection (0.33mg/kg LPS or Saline) we found a LPS-induced upregulation of whole brain TNFα, IL-1β, and IL-10 mRNA, and increased IL-1β and IL-6 in the spleen and liver; these effects were not attenuated by VWR. We conclude that VWR does not reduce LPS-induced exaggerated or prolonged sickness behavior in aged animals, or 24h post-injection (0.33mg/kg LPS or Saline) brain and peripheral proinflammatory cytokine gene expression. The necessity of the sickness response is critical for survival and may outweigh the subtle benefits of exercise training in aged animals.
登录
查看更多内容
DOI：
10.1139/h08-081
发表时间：
2008-10-01
期刊：
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM-PHYSIOLOGIE APPLIQUEE NUTRITION ET METABOLISME
影响因子：
--
作者：
Hung, Ching-Hsia;Chen, Yu-Wen;Cheng, Juei-Tang
通讯作者：
Cheng, Juei-Tang
DOI：
10.1523/jneurosci.5818-10.2011
发表时间：
2011-03-16
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cortese GP;Barrientos RM;Maier SF;Patterson SL
通讯作者：
Patterson SL
影响因子：
7.7
作者：
Oliveira AG;Carvalho BM;Tobar N;Ropelle ER;Pauli JR;Bagarolli RA;Guadagnini D;Carvalheira JB;Saad MJ
通讯作者：
Saad MJ
DOI：
10.1249/mss.0b013e31802d11c8
发表时间：
2007-03-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
Chen, Hsing I.;Hsieh, Shu-Ya;Lin, Chia-Chih
通讯作者：
Lin, Chia-Chih
影响因子：
15.1
作者：
Funk, Jason A.;Gohlke, Julia;Kraft, Andrew D.;McPherson, Christopher A.;Collins, Jennifer B.;Harry, G. Jean
通讯作者：
Harry, G. Jean