Corticosterone induces rapid spinogenesis via synaptic glucocorticoid receptors and kinase networks in hippocampus.
Corticosterone induces rapid spinogenesis via synaptic glucocorticoid receptors and kinase networks in hippocampus.
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皮质酮通过海马中的突触糖皮质激素受体和激酶网络诱导快速旋转生成。
DOI：
10.1371/journal.pone.0034124
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kawato S
中科院分区：
文献类型：
--
作者：
Komatsuzaki Y;Hatanaka Y;Murakami G;Mukai H;Hojo Y;Saito M;Kimoto T;Kawato S
树突棘在急性应激下的调控正引起人们的广泛关注。急性应激诱导肾上腺皮质分泌皮质酮（CORT），导致血浆和海马中CORT水平迅速升高。本文通过对成年雄性大鼠海马脑片神经元的成像，阐明了CORT对棘密度和形态的快速作用机制。应用100-1000 nM的CORT诱导CA 1锥体神经元棘密度的快速增加。即使在低CORT水平（100-200 nM）下，小头棘（0.2-0.4 µm）的密度也会增加。在400-1000 nM之间的高CORT水平下，中头棘（0.4-0.5 µm）的密度增加。大头棘（0.5-1.0 µm）的密度仅在1000 nM CORT时增加。同时给予糖皮质激素受体（GR）拮抗剂RU 486，可阻断CORT的作用。阻断单个激酶，如MAPK、PKA、PKC或PI 3 K，可抑制CORT诱导的棘生成增强。阻断NMDA受体可抑制CORT效应。这些结果暗示应激水平的CORT（100-1000 nM）通过突触GR和多个激酶途径驱动棘发生。
Modulation of dendritic spines under acute stress is attracting much attention. Exposure to acute stress induces corticosterone (CORT) secretion from the adrenal cortex, resulting in rapid increase of CORT levels in plasma and the hippocampus. Here we demonstrated the mechanisms of rapid effect (∼1 h) of CORT on the density and morphology of spines by imaging neurons in adult male rat hippocampal slices. The application of CORT at 100–1000 nM induced a rapid increase in the density of spines of CA1 pyramidal neurons. The density of small-head spines (0.2–0.4 µm) was increased even at low CORT levels (100–200 nM). The density of middle-head spines (0.4–0.5 µm) was increased at high CORT levels between 400–1000 nM. The density of large-head spines (0.5–1.0 µm) was increased only at 1000 nM CORT. Co-administration of RU486, an antagonist of glucocorticoid receptor (GR), abolished the effect of CORT. Blocking a single kinase, such as MAPK, PKA, PKC or PI3K, suppressed CORT-induced enhancement of spinogenesis. Blocking NMDA receptors suppressed the CORT effect. These results imply that stress levels of CORT (100–1000 nM) drive the spinogenesis via synaptic GR and multiple kinase pathways.
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DOI：
10.1016/0006-291x(90)91544-3
发表时间：
1990-11-15
影响因子：
3.1
作者：
HERBERT, JM;AUGEREAU, JM;MAFFRAND, JP
通讯作者：
MAFFRAND, JP
DOI：
10.1073/pnas.92.17.7686
发表时间：
1995-08-15
影响因子：
11.1
作者：
DUDLEY, DT;PANG, L;SALTIEL, AR
通讯作者：
SALTIEL, AR
影响因子：
25
作者：
Karst, H;Karten, YJG;Joëls, M
通讯作者：
Joëls, M
DOI：
10.1073/pnas.0337481100
发表时间：
2003-02-04
影响因子：
11.1
作者：
MacQueen, GM;Campbell, S;Young, LT
通讯作者：
Young, LT
影响因子：
3.9
作者：
Higashi, Eriko;Nakajima, Miki;Yokoi, Tsuyoshi
通讯作者：
Yokoi, Tsuyoshi